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Association of mtDNA D-Loop Polymorphisms with Risk of Gastric Cancer in Chinese Population

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Pathology & Oncology Research

Abstract

The aim of present study was to evaluate the association of common polymorphisms detected in mitochondrial DNA (mtDNA) D-loop region (mononucleotide repetitive D310, single nucleotide polymorphism (SNP) D16521) with susceptibility to gastric cancer (GC) in northwestern Chinese population. A total of 180 GC patients and 218 healthy controls were investigated by using PCR- denaturing high performance liquid chromatography (DHPLC) assay. Genotype and allele distributions and haplotype construction were analyzed in case–control study. We found D310 and D16521 heteroplasmy were significantly different between GC cases and controls (p < 0.05), and D16521 homoplasmy showed association with histological grade of GC (p < 0.05). Haplotype 7C/T, 8C/C and 9C/C had significant association with GC risk implied from analysis of D310 and D16521. Taken together, these findings suggested that mtDNA D-Loop polymorphisms and haplotypes may contribute to genetic susceptibility to GC in Chinese population.

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Abbreviations

GC:

Gastric cancer

MtDNA:

Mitochondrial DNA

SNP:

Single nucleotide polymorphism

D310:

Polytract of cytosines at nucleotide 310 in the mitochondrial genome

DHPLC:

Denaturing high performance liquid chromatography

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Acknowledgements

We express our appreciation to the original DNA donors who made this study possible. This work was supported by a grant from Natural Science Foundation of Gansu province (0710RJZA017), Key Technologies R&D Program (0708NKCA096) from the Gansu Provincial Science and Technology Committee, China, grant of Platform of Genetic Resource of Chinese Project, from the Ministry of Science and Technology PR China (2006DKA21301), and Program for New Century Excellent Talents in University, from the Ministry of Education of PR China (NCET-05-0885), National Natural Science Foundation of China (81071701), and State Key Development Program of Basic Research of China (2010CB834201).

Conflicts of interest

No conflicts of interest were declared in relation to this article.

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Authors and Affiliations

  1. Key Laboratory of Preclinical Study for New Drugs of Gansu Province, school of Basic Medical Science, Lanzhou University, 199 West DongGang Road, Lanzhou, Gansu Province, 730000, China

    Pei-qiang Li, Hua Wu & Xiao-dong Xie

  2. School of Life Science, Lanzhou University, Lanzhou, Gansu Province, China

    Li Wei, Ke-jun Ma & Xiao-dong Xie

  3. General Hospital of Lanzhou Command, PLA, Lanzhou, 730050, China

    Yong Zhao & Feng Gao

  4. Surgical Department, People’s Hospital of Gansu Province, Lanzhou, 730000, Gansu Province, China

    Li Wei & Tian-kang Guo

  5. School of Basic Medical Sciences & Forensic Sciences, Sichuan University, Chengdu, 610041, China

    Han-bing Xie

  6. Surgical Department, Lanzhou General Hospital of Lanzhou Command, PLA, Lanzhou, 730050, China

    Feng Gao

Authors
  1. Li Wei
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  2. Yong Zhao
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  3. Tian-kang Guo
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  4. Pei-qiang Li
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  5. Hua Wu
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  7. Ke-jun Ma
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  8. Feng Gao
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  9. Xiao-dong Xie
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Corresponding authors

Correspondence to Feng Gao or Xiao-dong Xie.

Additional information

Li Wei and Yong Zhao contributed equally to this work.

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Wei, L., Zhao, Y., Guo, Tk. et al. Association of mtDNA D-Loop Polymorphisms with Risk of Gastric Cancer in Chinese Population. Pathol. Oncol. Res. 17, 735–742 (2011). https://doi.org/10.1007/s12253-011-9378-7

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  • DOI: https://doi.org/10.1007/s12253-011-9378-7

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