Abstract
Hubei Province is a major epidemic area of severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) in China. However, to date, a few SFTSV strains have been isolated from Hubei Province, preventing effective studies of epidemic outbreaks. Here, we report three confirmed patients (2015–2016) with typical symptoms of severe fever with thrombocytopenia syndrome disease (SFTS) who were farmers resident in different regions in Hubei Province. Three new SFTSV strains were isolated from the serum samples of each patient. Characterization of viral growth properties showed that there were no significant differences in virus production. All strains were completely sequenced, and phylogenetic analysis showed that unlike the other strains from Hubei province, which belonged to the SFTSV C3 genotype, one of the three strains belonged to the SFTSV C2 genotype. These results suggested that multiple SFTSV genotypes have been circulating in Hubei Province, providing insights into SFTSV evolution and improving our understanding of SFTSV prevalence in Hubei Province.
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Acknowledgments
This work was supported by the Science and Technology Basic Work Program (2013FY113500) and the National Key Research and Development Program (2016YFE 0113500) from the Ministry of Science and Technology of China, as well as the European Union’s Horizon 2020 EVAg project (No 653316).
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ORCID: 0000-0002-5385-083X
ORCID: 0000-0003-1209-5281
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The authors declare that they have no conflict of interest. The study was approved by both ethics committees of Wuhan Institute of Virology, Chinese Academy of Sciences (Approval number: WIVH332011601), and the Union Hospital, Tongji Medical College, HuaZhong University of Science and Technology (Approval number: 2015LSZ[031]). All participants provided written consent for anonymous use of their specimens and clinical information for research.
Author Contributions
ZHH, XZ, FD, and SS conceived of the study. YFZ and SS isolated and identified the SFTSV and wrote the manuscript. XZ, CP, MYL, WJZ, and MML collected the serum samples and patient information and performed diagnostic testing and treatment. ZYS obtained the full-length SFTSV sequences. JMS generated the sequence datasets for phylogenetic analysis and constructed the phylogenetic trees. ZHH, XZ, and FD checked and finalized the manuscript.
Supplementary Table S1 is available on the websites of Virologica Sinica: www.virosin.org; link.springer.com/journal/12250.
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Isolation, characterization, and phylogenic analysis of three new severe fever with thrombocytopenia syndrome bunyavirus strains derived from Hubei Province, China
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Zhang, Y., Shen, S., Shi, J. et al. Isolation, characterization, and phylogenic analysis of three new severe fever with thrombocytopenia syndrome bunyavirus strains derived from Hubei Province, China. Virol. Sin. 32, 89–96 (2017). https://doi.org/10.1007/s12250-017-3953-3
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DOI: https://doi.org/10.1007/s12250-017-3953-3