Skip to main content
SpringerLink
Log in

Phylogenetic analysis of Orgyia pseudotsugata single-nucleocapsid nucleopolyhedrovirus

  • Published:
Virologica Sinica

Abstract

The Douglas-fir tussock moth Orgyia pseudotsugata (Lepidoptera: Lymantriidae) is a frequent defoliator of Douglas-fir and true firs in western USA and Canada. A single nucleopolyhedrovirus (SNPV) isolated from O. pseudotsugata larvae in Canada (OpSNPV) was previously analyzed via its polyhedrin gene, but is phylogenetic status was ambiguous. Sequences of four conserved baculovirus genes, polyhedrin, lef-8, pif-2 and dpol, were amplified from OpSNPV DNA in polymerase chain reactions using degenerate primer sets and their sequences were analyzed phylogenetically. The analysis revealed that OpSNPV belongs to group II NPVs and is most closely related to SNPVs that infect O. ericae and O. anartoides, respectively. These results show the need for multiple, concatenated gene phylogenies to classify baculoviruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ahrens C H, Russell R L, Funk C J, et al. 1997. The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. Virology, 229: 381–399.

    Article  PubMed  CAS  Google Scholar 

  2. Altschul S F, Gish W, Miller W, et al. 1990. Basic local alignment search tool. J Mol Biol, 215: 403–410.

    PubMed  CAS  Google Scholar 

  3. Beckwith R C. 1978. Biology of the insect. In: The Douglas-fir Tussock Moth: A Synthesis. United States Department of Agriculture Forest Service Technical Bulletin 1585 (Brookes M H, Stark R W, Campbell R W, eds.). Washington DC, 25–30.

  4. Bulach D M, Kumar C A, Zaia A, et al. 1999. Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J Invert Pathol, 73: 59–73.

    Article  CAS  Google Scholar 

  5. Herniou E A. 2003. Use of comparative genomics and phylogenetics to study the evolution of the Baculoviridae (PhD Thesis). Centre for Ecology & Hydrology Imperial College London UK

    Google Scholar 

  6. Herniou E A, Luque T, Chen X, et al. 2001. Use of whole genome sequence data to infer baculovirus phylogeny. J Virol, 75: 8117–8126.

    Article  PubMed  CAS  Google Scholar 

  7. Herniou E A, Olszewski J A, O’Reilly D R, et al. 2004. Ancient coevolution of baculoviruses and their insect hosts. J Virol, 78: 3244–3251.

    Article  PubMed  CAS  Google Scholar 

  8. Hughes K M. 1976. Notes on the nuclear polyhedrosis viruses of tussock moths of the genus Orgyia (Lepidoptera). Can Entomol, 108: 479–484.

    Article  Google Scholar 

  9. Hughes K M, Addison R B. 1970. Two nuclear polyhedrosis viruses of the Douglas-fir tussock moth. J Invert Pathol, 16: 196–204.

    Article  Google Scholar 

  10. IJkel W F, Westenberg M, Goldbach R W, et al. 2000. A novel baculovirus envelope protein with a proprotein convertase cleavage site. Virology, 275: 30–41.

    Article  PubMed  CAS  Google Scholar 

  11. Jakubowska A, Oers M M, Cory J S, et al. 2005. European Leucoma salicis MNPV is closely related to Orgyia pseudotsugata MNPV of North-America. J Invert Pathol, 88: 100–107.

    Article  CAS  Google Scholar 

  12. Jehle J A. 2004. The mosaic structure of the polyhedrin gene of the Autographa californica nucleopolyhedrovirus (Ac-MNPV). Virus Genes, 29: 5–8.

    Article  PubMed  CAS  Google Scholar 

  13. Jehle J A, Blissard G W, Bonning B C, et al. 2006. On the classification and nomenclature of baculoviruses: a proposal for revision. Arch Virol, 151: 1257–1266.

    Article  PubMed  CAS  Google Scholar 

  14. Jehle J A, Lange M, Wang H, et al. 2006. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology, 346: 180–193.

    Article  PubMed  CAS  Google Scholar 

  15. Kumar S, Tamura K, Nei M. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinf, 5: 150–163.

    Article  CAS  Google Scholar 

  16. Lange M, Wang H, Zhihong H, et al. 2004. Towards a molecular identification and classification system of lepidopteran-specific baculoviruses. Virology, 325: 36–47.

    Article  PubMed  CAS  Google Scholar 

  17. Leisy D, Nesson M, Pearson M, et al. 1986. Location and nucleotide sequence of the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus polyhedrin gene. J Gen Virol, 67: 1073–1079.

    Article  PubMed  CAS  Google Scholar 

  18. Leisy D, Rohrmann G, Beaudreau G. 1986. The nucleotide sequence of the polyhedrin gene region from the multicapsid baculovirus of Orgyia pseudotsugata. Virology, 153: 280–288.

    Article  PubMed  CAS  Google Scholar 

  19. Moraes R R, Maruniak J E. 1997. Detection and identification of multiple baculoviruses using the polymerase chain reaction (PCR) and restriction endonuclease analysis. J Virol Methods, 63: 209–217.

    Article  PubMed  Google Scholar 

  20. Moscardi F. 1999. Assessment of the application of baculoviruses for control of Lepidoptera. Annu Rev Entomol, 44: 257–289.

    Article  PubMed  CAS  Google Scholar 

  21. Pearson M, Groten C, Rohrmann G F. 2000. Identification of the Lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of Baculoviridae. J Virol, 74: 6126–6131.

    Article  PubMed  CAS  Google Scholar 

  22. Reed C, Otvos I S, Reardon R, et al. 2003. Effects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1. J Invert Pathol, 84: 104–113.

    Article  CAS  Google Scholar 

  23. Richards A, Speight M, Cory J. 1999. Characterization of a nucleopolyhedrovirus from the vapourer moth Orgyia antiqua (Lepidoptera Lymantriidae). J Invert Pathol, 74: 137–142.

    Article  CAS  Google Scholar 

  24. Rohrmann G F, Beaudreau G S. 1977. Characterization of DNA from polyhedral inclusion bodies of the nucleopolyhedrosis single-rod virus pathogenic for Orgyia pseudotsugata. Virology, 83: 474–478.

    Article  PubMed  CAS  Google Scholar 

  25. Swofford D L. 1998. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Associates Sunderland M A.

    Google Scholar 

  26. Theilmann D A, Blissard G W, Bonning B, et al. 2005. Family Baculoviridae. In: Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses (Fauquet C M, Mayo M A, Maniloff J, Desselberger U, Ball L A. eds.). Academic Press San Diego California, 177–185.

    Google Scholar 

  27. Vlak J M, Rohrmann G F. 1985. The nature of polyhedrin. In: Viral insecticides for biological control (Maramorosh K and Sherman K E eds). Academic Press Orlando, 489–544.

    Google Scholar 

  28. Walsh P J, Glare T R, Nelson T L, et al. 1999. Virulence of nucleopolyhedroviruses from Orgyia pseudotsugata and Orgyia leucostigma (Lep. Lymantriidae) for early instars of the white-spotted tussock moth Orgyia thyellina. J Appl Entomol, 123: 375–379.

    Article  CAS  Google Scholar 

  29. Yang L R, Wang D, Duan L Q, et al. 2006. Polyhedrin gene sequence and phylogenetic analysis of a nucleopolyhedrovirus isolated from Orgyia ericae Germar. DNA Sequence, 17: 215–222.

    Article  PubMed  CAS  Google Scholar 

  30. Zanotto P M, Kessing B D, Maruniak J E. 1993. Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. J Invert Pathol, 62: 147–164.

    Article  CAS  Google Scholar 

  31. Zhang G Y, Sun X L, Zhang Z X, et al. 1995. Production and effectiveness of the new formulation of Helicoverpa virus pesticide-emulsifiable suspension. Virologica Sinica, 10: 242–247.

    Google Scholar 

  32. Ziemnicka J. 2000. Rola bulowirusa SsMNPV w regulacji liczebnosci populacji bialki wierbowki Stilpnotia salicis L. (Lepidoptera: Lymantriidae). (in Polish). Rozprawy Naukowe Instytut Ochrony Roslin, Zeszyt 6: 85

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Virology, Wageningen University, Binnenhaven 11, Wageningen, 6709 PD, The Netherlands

    Agata Jakubowska, Monique M. van Oers & Just M. Vlak

  2. Department of Biological Control and Quarantine, Institute of Plant Protection, Miczurina 20, Poznan, 60-318, Poland

    Agata Jakubowska

  3. Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, BC V8Z 1M5, Victoria, Canada

    Imre S. Otvos

Authors
  1. Agata Jakubowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Monique M. van Oers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Imre S. Otvos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Just M. Vlak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Just M. Vlak.

Additional information

Foundation item: Supported by a scholarship from the European Union (Functional Biodiversity and Crop Protection), contract no HPMT-CT-2000-00199.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jakubowska, A., van Oers, M.M., Otvos, I.S. et al. Phylogenetic analysis of Orgyia pseudotsugata single-nucleocapsid nucleopolyhedrovirus. Virol. Sin. 22, 257–265 (2007). https://doi.org/10.1007/s12250-007-0001-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12250-007-0001-8

Key words

CLC number

Search

Navigation