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Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus

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Virologica Sinica

Abstract

The Cyclophragma undans nucleopolyhedrovirus (CyunNPV), a potential pest control agent, was isolated from Cyclophragma undans (Lepidoptera: Lasiocampidae), an important forest pest. In the present study, we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group I alphabaculoviruses. Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G + C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group I Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to CyunNPV. In addition, the genome contains 13 homologous repeated sequences (hrs). Phylogenetic analysis groups CyunNPV under a distinct branch within clade “a” of Group I in the genus Alphabaculovirus. Unlike other members of Group I, CyunNPV harbors only nine of the 11 genes previously determined to be specific to Group I viruses. Furthermore, the CyunNPV lacks the tyrosine phosphatase gene and the ac30 gene. The CyunNPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pre-transmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings imply that CyunNPV is a distinct species under Group I Alphabaculovirus.

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Acknowledgements

This work was supported by the National Key R&D Program of China (Grant No. 2017YFD0200400) and the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB11030400). We acknowledge the technical support and core facility of the Wuhan Institute of Virology for their technical assistance.

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Authors and Affiliations

  1. State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Zheng Zhu, Jun Wang, Qianran Wang, Feifei Yin, Xiaoping Liu, Dianhai Hou, Lei Zhang, Haizhou Liu, Jiang Li, Hualin Wang, Fei Deng, Zhihong Hu & Manli Wang

  2. Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste Marie, ON, P6A 2E5, Canada

    Basil M. Arif

Authors
  1. Zheng Zhu
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  2. Jun Wang
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Contributions

Conceived and designed the experiments: ZZ, MW, ZH, FD, HW, and FY. Viral nucleic acid extraction and 454 sequencing: ZZ, DH, FY, XL, and ZL. Genome assembly, annotation and data analysis: ZZ, XL, JW, QW, and HL. Wrote the paper: ZZ, BA, ZH, and MW.

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Correspondence to Manli Wang.

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Zhu, Z., Wang, J., Wang, Q. et al. Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus. Virol. Sin. 33, 359–368 (2018). https://doi.org/10.1007/s12250-018-0047-9

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