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High Levels of Heterozygosity Found for 15 SSR Loci in Solanum chacoense

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Abstract

Wild species-related germplasm is widely used to introduce new alleles and/or increase heterozygosity in cultivated species. Twenty-four SSR markers, specifically designed for cultivated potatoes, were evaluated to determine the extent of genetic variation within and among ten accessions of Solanum chacoense (chc). Fifteen of these markers were informative: there was no polymorphism in one of the markers, four of the markers showed evidence that more than one locus was being amplified, and the other four markers failed to consistently amplify products. Heterozygosity in these 10 accessions ranged from 33% to 87%. Variation among accessions was the largest proportion of variance for three markers, variation among genotypes within accessions was the largest proportion for three markers, and for the other nine markers variation within genotypes (chromosome to chromosome) was the largest proportion. Genetic similarity averaged 29.5% across markers. Where accessions have already been screened and found to possess the trait of interest, multiple genotypes from those accessions should be evaluated to identify genotypes with the greatest expression of the trait.

Resumen

El germoplasma relacionado a especies silvestres se usa ampliamente para introducir nuevos alelos y/o aumentar la heterozigocidad en especies cultivadas. Se evaluaron 24 marcadores SSR específicamente diseñados para papas cultivadas, para determinar la amplitud de la variación genética dentro y entre diez introducciones de Solanum chacoense (chc). Quince de estos marcadores fueron informativos: no hubo polimorfismo en uno de los marcadores, cuatro de ellos mostraron evidencia de que más de un locus estaba siendo amplificado, y los otros cuatro marcadores fallaron para amplificar consistentemente los productos. La eterozigocidad en estas diez introducciones varió de 33% a 87%. La variación entre las accesiones fue la proporción mas grande de varianza para tres marcadores, la variación entre genotipos dentro de las introducciones fue la de mayor proporción para tres marcadores, y para los otros nueve la variación dentro de genotipos (cromosoma a cromosoma) fue la proporción más grande. La similitud genética promedió 29.5% entre los marcadores. En donde las accesiones ya han sido analizadas y que se encontró que poseen el carácter de interés, múltiples genotipos de estas introducciones deberían evaluarse para identificar genotipos con la mayor expansión del carácter.

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Acknowledgements

The authors are grateful to Drs. Benildo de los Reyes and James Olmstead for their insights and advice on the project. We also thank Dr. John Bamberg and Max Martin at the Potato Gene Bank for experimental material. Thanks, too, to Dr. Bamberg for his many helpful suggestions to improve the manuscript.

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  1. Genetic Improvement of Fruits and Vegetables Laboratory, U.S.D.A.-Agricultural Research Service, Beltsville, MD, 20705, USA

    Kathleen G. Haynes, Haitham E. M. Zaki, Elizabeth Ogden & Lisa J. Rowland

  2. Horticulture Department, Faculty of Agriculture, Minia University, El-Minia, 61517, Egypt

    Haitham E. M. Zaki

  3. Horticultural Sciences Department, University of Florida, Gainesville, FL, 32611, USA

    Christian T. Christensen & Lincoln Zotarelli

  4. Statistics Group, U.S.D.A.-Agricultural Research Service, Beltsville, MD, 20705, USA

    Matthew Kramer

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Haynes, K.G., Zaki, H.E.M., Christensen, C.T. et al. High Levels of Heterozygosity Found for 15 SSR Loci in Solanum chacoense . Am. J. Potato Res. 94, 638–646 (2017). https://doi.org/10.1007/s12230-017-9602-4

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