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Glutamine synthetase type I (glnAI) represents a rewarding molecular marker in the classification of bifidobacteria and related genera

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Abstract

The family Bifidobacteriaceae constitutes an important phylogenetic group that particularly includes bifidobacterial taxa demonstrating proven or debated positive effects on host health. The increasingly widespread application of probiotic cultures in the twenty-first century requires detailed classification to the level of particular strains. This study aimed to apply the glutamine synthetase class I (glnAI) gene region (717 bp representing approximately 50% of the entire gene sequence) using specific PCR primers for the classification, typing, and phylogenetic analysis of bifidobacteria and closely related scardovial genera. In the family Bifidobacteriaceae, this is the first report on the use of this gene for such purposes. To achieve high-value results, almost all valid Bifidobacteriaceae type strains (75) and 15 strains isolated from various environments were evaluated. The threshold value of the glnAI gene identity among Bifidobacterium species (86.9%) was comparable to that of other phylogenetic/identification markers proposed for bifidobacteria and was much lower compared to the 16S rRNA gene. Further statistical and phylogenetic analyses suggest that the glnAI gene can be applied as a novel genetic marker in the classification, genotyping, and phylogenetic analysis of isolates belonging to the family Bifidobacteriaceae.

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Funding

This study was funded by the Project Excellence (no. CZ.02.1.01/0.0/0.0/15_003/0000460), the Czech National Agency for Agricultural Research (project no. QJ1510338), and the Czech Health Research Council (project no. 16-27449A).

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Authors and Affiliations

  1. Institute of Animal Physiology and Genetics, v.v.i., Czech Academy of Sciences, Vídeňská 1083, 142 20, Prague 4, Krč, Czechia

    Jiří Killer, Chahrazed Mekadim & Jakub Mrázek

  2. Faculty of Agrobiology, Food and Natural Resources, Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, Kamýcká 129, 165 00, Prague 6, Suchdol, Czechia

    Jiří Killer, Chahrazed Mekadim, Věra Bunešová, Zuzana Hroncová & Eva Vlková

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  1. Jiří Killer
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  2. Chahrazed Mekadim
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  3. Věra Bunešová
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  4. Jakub Mrázek
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  5. Zuzana Hroncová
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  6. Eva Vlková
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Correspondence to Jiří Killer.

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Killer, J., Mekadim, C., Bunešová, V. et al. Glutamine synthetase type I (glnAI) represents a rewarding molecular marker in the classification of bifidobacteria and related genera. Folia Microbiol 65, 143–151 (2020). https://doi.org/10.1007/s12223-019-00716-0

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