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Homologous constitutive expression of Xyn III in Trichoderma reesei QM9414 and its characterization

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Abstract

Xylanase III (Xyn III), a specific endoxylanase that belongs to family 10 of the glycoside hydrolases, was overexpressed in Trichoderma reesei QM9414 using a constitutive strong promoter of the gene encoding pyruvate decarboxylase (pdc). The maximum recombinant xylanase activity achieved was 817.2 ± 65.2 U/mL in the transformant fermentation liquid. The productivities of Xyn III accounted for approximately 53 % of the total protein secreted by the recombinant. The enzyme was optimally active at 60 °C and pH 6. The recombinant Xyn III was stable at pH 5–8. This is the first report on the homologous expression of xyn3 in T. reesei QM9414. The properties of Xyn III make it promising in a variety of industrial use.

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Acknowledgments

This work is supported by the Shenzhen Municipal Science and Technology Basic Research Program (JC201005280398A) and Shenzhen Municipal Science and Technology key projects of the Basic Research Program (JC201005250041A).

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Authors and Affiliations

  1. Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong, 518060, People’s Republic of China

    Juan Wang, Gang Liu & Shaowen Yu

  2. Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen, Guangdong, 518060, People’s Republic of China

    Desheng Zeng

  3. Research Center for Biomedical Information Technology, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, People’s Republic of China

    Guoqin Mai

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  1. Juan Wang
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  2. Desheng Zeng
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  3. Guoqin Mai
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  4. Gang Liu
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  5. Shaowen Yu
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Correspondence to Shaowen Yu.

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Wang, J., Zeng, D., Mai, G. et al. Homologous constitutive expression of Xyn III in Trichoderma reesei QM9414 and its characterization. Folia Microbiol 59, 229–233 (2014). https://doi.org/10.1007/s12223-013-0288-9

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  • DOI: https://doi.org/10.1007/s12223-013-0288-9

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