Abstract
Eleven type strains and 24 Lactobacillus isolates, preliminarily classified to the species due to phenotypic features, were investigated. Standard methods of identification with species-specific PCRs and typing with PFGE (with ApaI, NotI and SmaI restriction enzymes) allowed us to distinguish 16 unique strains belonging to 5 species (L. acidophilus, L. delbrueckii ssp. bulgaricus, L. plantarum, L. rhamnosus, L. salivarius). Alternative approach with 16S–23S rDNA ARDRA identification (with merely two restrictases, BsuRI and TaqI) and PCR-based typing (RAPD with two random- and rep-PCR with (GTG)5 primers) showed to be more discriminative, i.e. 21 unique strains were classified in the same species as above. As a result, 7 out of 24 phenotypically species-assigned isolates were reclassified. The alternative procedure of rapid identification and typing of Lactobacillus isolates appeared to be equally effective and shortened from 1 week to 2–3 d (in comparison to the standard methods).
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Abbreviations
- ARDRA:
-
amplified ribosomal DNA restriction analysis
- CFU:
-
colony forming units
- DSM:
-
Deutsche Sammlung von Mikroorganismen
- PCR(s):
-
polymerase chain reaction(s)
- PFGE:
-
pulsed-field gel electrophoresis
- RAPD:
-
randomly amplified polymorphic DNA
- rep-PCR:
-
PCR of repetitive bacterial DNA
- UPGMA:
-
unweighted pair group method with arithmetic mean
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Markiewicz, L.H., Biedrzycka, E., Wasilewska, E. et al. Rapid molecular identification and characteristics of Lactobacillus strains. Folia Microbiol 55, 481–488 (2010). https://doi.org/10.1007/s12223-010-0080-z
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DOI: https://doi.org/10.1007/s12223-010-0080-z