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RAPD and rep-PCR fingerprinting for characterization of Bifidobacterium species

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Abstract

DNA fingerprinting methods, RAPD with 7 random primers, and rep-PCR using both BOXA1R and (GTG)5 ones, were used for the discrimination of 16 type and collection Bifidobacterium strains of 9 species of human origin, B. animalis ssp. animalis and B. animalis ssp. lactis and 7 Bifidobacterium strains collected in the Culture Collection of Dairy Microorganisms (CCDM). Both RAPD and rep-PCR methods provided similar results. The strains were identified as B. animalis ssp. lactis (6 strains) and B. adolescentis (1 strain). The reclassification of the collection strain CCM 3761 as B. pseudocatenulatum species (previously classified as B. adolescentis) was confirmed.

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Abbreviations

ARDRA:

amplified ribosomal DNA restriction analysis

ATCC:

American Type Culture Collection

CCDM:

Culture Collection of Dairy Microorganisms

CCM:

Czech Collection of Microorganisms

DSM:

Deutsche Sammlung von Mikroorganismen

DSMZ:

Deutsche Sammlung von Mikroorganismen und Zellkulturen

RAPD:

random amplified polymorphic DNA

UPGMA:

unweighted pair group method with arithmetic mean

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Authors and Affiliations

  1. Institute of Experimental Biology, Faculty of Science, Masaryk University, 602 00, Brno, Czechia

    J. Křížová, A. Španová & B. Rittich

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  1. J. Křížová
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  2. A. Španová
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  3. B. Rittich
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Correspondence to B. Rittich.

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Křížová, J., Španová, A. & Rittich, B. RAPD and rep-PCR fingerprinting for characterization of Bifidobacterium species. Folia Microbiol 53, 99–104 (2008). https://doi.org/10.1007/s12223-008-0014-1

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  • DOI: https://doi.org/10.1007/s12223-008-0014-1

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