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Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools

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Abstract

Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools and in healthy exclusively breast-fed babies was compared. Total anaerobes, bifidobacteria, lactobacilli, coliform bacteria, enterococci and clostridia were quantified by cultivation methods in feces of 17 full-term exclusively breastfed patients (aged 16.3 ± 7.4 weeks) with blood-streaked stools and in the control group of 22 healthy fullterm exclusively breast-fed infants (13.7 ± 6.4 weeks). Specific fluorescence in situ hybridization kits for Bifidobacterium spp. were used for the quantitative detection of bifidobacteria in samples. Control samples had significantly (p < 0.05) higher counts of total anaerobes. Bifidobacteria were not detected in patients’ samples in 65 % and in controls in 36 % (p < 0.01). Bifidobacteria counts were also significantly higher in the control group (p < 0.01). Furthermore, clostridia strains were detected only in feces from bifidobacteria-negative infants reaching counts >8 log CFU/g. Lactobacilli were not detected in 65 % patients and in 45 % control samples. However, this difference was not significant as well as the difference in lactobacilli counts. Eosinophilia was observed in 35 % of patients, low IgA concentration in 71 % and also low IgG concentration in 71 %. pANCA positivity was found in 53 % of patients. In conclusion a significant low proportion of bifidobacterial microbiota in patients with blood-streaked stools was shown in comparison with controls.

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Abbreviations

ANA:

antinuclear antibody

CRP:

C-reactive protein

ANCA:

antineutrophil cytoplasmic antibodies

ENA:

extractable nuclear antigens

ASCA:

antibodies to Saccharomyces cerevisiae

FISH:

fluorescence in situ hybridization

C3, C4:

complement components

IgG, IgA, IgM, IgE:

immunoglobulins G, A, M, E

CFU:

colony forming units

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Authors and Affiliations

  1. Department of Pediatrics, 2nd Medical School of the Charles University in Prague and University Hospital Motol, 150 06, Prague, Czech Republic

    J. Nevoral, K. Bláhová & J. Bronský

  2. Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences in Prague, Prague, Czech Republic

    V. Rada, E. Vlková & J. Killer

  3. Primary Care Pediatrician, Health Centre Prague 10, Prague, Czech Republic

    D. Bubáková

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  1. J. Nevoral
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  2. V. Rada
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  3. E. Vlková
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  4. K. Bláhová
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  5. J. Bronský
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  6. D. Bubáková
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  7. J. Killer
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Correspondence to J. Nevoral.

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Nevoral, J., Rada, V., Vlková, E. et al. Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools. Folia Microbiol 54, 167–171 (2009). https://doi.org/10.1007/s12223-009-0026-5

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  • DOI: https://doi.org/10.1007/s12223-009-0026-5

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