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Lactobacillus isolates from weaned piglets’ mucosa with inhibitory activity against common porcine pathogens

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Abstract

Twelve lactobacilli isolates from mucosa of 3–5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (S. aureus, B. cereus, E. coli, C. perfringens). Two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. Isolates 4/26 and 2/25 (identified as L. crispatus or L. amylovorus) were inhibitory against most strains of S. aureus, B. cereus and E. coli, and especially the strain 4/26 survived well in simulated gastric and intestinal juice. Diarrhea-causing E. coli O8K88H9 Ent+ was successfully inhibited by the growing culture as well as by the catalase-treated and neutralized supernatant of L. reuteri 12/26. Mucin degradation and multiple resistance to antibiotics were not observed.

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Abbreviations

ETEC:

enterotoxigenic E. coli

LAB:

lactic acid bacteria

MRS:

de Man-Rogosa-Sharp (agar)

GI:

gastrointestinal

MHA:

Mueller-Hinton agar

VFAs:

volatile fatty acids

HGM:

hog gastric mucin

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Authors and Affiliations

  1. Department of Animal Science, Biotechnical Faculty, University of Ljubljana, SI-1230, Domžale, Slovenia

    B. Hacin, I. Rogelj & B. B. Matijašić

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  1. B. Hacin
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  2. I. Rogelj
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  3. B. B. Matijašić
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Correspondence to B. B. Matijašić.

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Hacin, B., Rogelj, I. & Matijašić, B.B. Lactobacillus isolates from weaned piglets’ mucosa with inhibitory activity against common porcine pathogens. Folia Microbiol 53, 569–576 (2008). https://doi.org/10.1007/s12223-008-0091-1

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  • DOI: https://doi.org/10.1007/s12223-008-0091-1

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