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Detection of possible AI-2-mediated quorum sensing system in commensal intestinal bacteria

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Abstract

The Vibrio harveyi strain BB170-autoinducer bioassay was used to detect possible quorum sensing autoinducer-2 molecule (AI-2) in culture fluids of commensal intestinal bacteria. Culture fluids of Bacteroides vulgatus, Clostridium proteoclasticum, Escherichia coli, Eubacterium rectale, Lachnospira multipara, Pseudobutyrivibrio ruminis, Roseburia intestinalis, Ruminococcus albus and Ruminococcus flavefaciens contained AI-2-like molecules. The PCR bands from some of the tested strains could be also amplified using primers designed for the luxS gene. These findings suggest that AI-2 is present in the gastrointestinal tract; however, it has not yet been proved whether it is used for bacterial cell-to-cell communication.

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Authors and Affiliations

  1. Institute of Animal Physiology and Genetics, v.v.i., Academy of Science of the Czech Republic, 142 20, Prague, Czechia

    F. Lukáš & J. Kopečný

  2. Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    G. Gorenc

Authors
  1. F. Lukáš
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  2. G. Gorenc
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  3. J. Kopečný
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Correspondence to F. Lukáš.

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Lukáš, F., Gorenc, G. & Kopečný, J. Detection of possible AI-2-mediated quorum sensing system in commensal intestinal bacteria. Folia Microbiol 53, 221–224 (2008). https://doi.org/10.1007/s12223-008-0030-1

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  • DOI: https://doi.org/10.1007/s12223-008-0030-1

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