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In-situ and highly sensitive detection of epidermal growth factor receptor mutation using nano-porous quartz crystal microbalance

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Abstract

Assessment of epidermal growth factor receptor (EGFR) exon 19 mutations is very important indicator for treating lung cancer patients. In this report, we propose in-situ and highly sensitive detection method of EGFR mutation using quartz crystal microbalance (QCM) and nano-porous structured QCM electrode. The detection is based on the resonance frequency shift of QCM electrode upon DNA hybridization between probe DNA and EGFR mutant DNA (746–750 (del ELREA) in EGFR exon 19). Nano-porous structure is produced on the QCM electrode through simple processes of electrochemical deposition and etching. By using QCM, in-situ detection of EGFR mutation is performed within 30 mins. High sensitivity is achieved by introducing nano-porous structure on a QCM electrode, and the limit of detection (LOD) is 1 nM.

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Authors and Affiliations

  1. Department of Mechanical Engineering, Korea University, Seoul, 02841, Korea

    Hyunjun Park, Juneseok You, Chanho Park & Sungsoo Na

  2. School of Mechanical Engineering, Hoseo University, Asan, 31499, Korea

    Kuewhan Jang

Authors
  1. Hyunjun Park
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  2. Juneseok You
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  3. Chanho Park
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  4. Kuewhan Jang
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  5. Sungsoo Na
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Corresponding authors

Correspondence to Kuewhan Jang or Sungsoo Na.

Additional information

Recommended by Associate Editor Won Hyoung Ryu

Sungsoo Na is a Professor of Department of Mechanical Engineering at Korea University since 2001. He obtained his Ph.D. in 1997 from Virginia Tech in USA. He is interested in Sensing of Circulator Tumor DNA and Nanotoxic material.

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Park, H., You, J., Park, C. et al. In-situ and highly sensitive detection of epidermal growth factor receptor mutation using nano-porous quartz crystal microbalance. J Mech Sci Technol 32, 1927–1932 (2018). https://doi.org/10.1007/s12206-018-0348-9

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  • DOI: https://doi.org/10.1007/s12206-018-0348-9

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