Abstract
Small heat shock proteins (Hsps) protect against stress-inducible denaturation of substrates. Our objectives were to clone and examine the mRNA expression of the Hsp16.9 gene from Siberian wild rye grown under diverse stress treatments. We characterized EsHsp16.9 from Elymus sibiricus L. EsHsp16.9 has a 456-bp open reading frame that encodes a 151-amino acid protein with a conserved α-crystallin domain. Northern blot analysis showed that EsHsp16.9 transcripts were enhanced by heat, drought, arsenate, methyl viologen, and H2O2 treatment. In addition, recombinant EsHsp16.9 protein acts as a molecular chaperone to prevent the denaturation of malate dehydrogenase. Growth of cells overexpressing EsHsp16.9 was up to 200 % more rapid in the presence of NaCl, arsenate, and polyethylene glycol than that of cells harboring an empty vector. These data suggest that EsHsp16.9 acts as a molecular chaperone that enhances stress tolerance in living organisms.
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Acknowledgments
This study was carried out with the support of the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ008599042015)” of National Institute of Animal Science, Rural Development Administration, Republic of Korea.
Supporting information
Supplementary Figure 1—Nucleotide and deduced amino acid sequences of small Hsp16.9 (EsHsp16.9) from Siberian wild rye (Elymus sibiricus L).
Supplementary Figure 2—Northern blot analysis of EsHsp16.9 transcripts in response to abiotic stressors.
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Sang-Hoon Lee and Ki-Won Lee have contributed equally to the work.
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Lee, SH., Lee, KW., Lee, DG. et al. Identification and functional characterization of Siberian wild rye (Elymus sibiricus L.) small heat shock protein 16.9 gene (EsHsp16.9) conferring diverse stress tolerance in prokaryotic cells. Biotechnol Lett 37, 881–890 (2015). https://doi.org/10.1007/s10529-014-1749-1
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DOI: https://doi.org/10.1007/s10529-014-1749-1