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Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity

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Cell Stress and Chaperones Aims and scope

Abstract

Extracellular heat-shock protein 72 (eHsp72) expression during exercise-heat stress is suggested to increase with the level of hyperthermia attained, independent of the rate of heat storage. This study examined the influence of exercise at various intensities to elucidate this relationship, and investigated the association between eHsp72 and eHsp27. Sixteen male subjects cycled to exhaustion at 60% and 75% of maximal oxygen uptake in hot conditions (40°C, 50% RH). Core temperature, heart rate, oxidative stress, and blood lactate and glucose levels were measured to determine the predictor variables associated with eHsp expression. At exhaustion, heart rate exceeded 96% of maximum in both conditions. Core temperature reached 39.7°C in the 60% trial (58.9 min) and 39.0°C in the 75% trial (27.2 min) (P < 0.001). The rate of rise in core temperature was 2.1°C h−1 greater in the 75% trial than in the 60% trial (P < 0.001). A significant increase and correlation was observed between eHsp72 and eHsp27 concentrations at exhaustion (P < 0.005). eHsp72 was highly correlated with the core temperature attained (60% trial) and the rate of increase in core temperature (75% trial; P < 0.05). However, no common predictor variable was associated with the expression of both eHsps. The similarity in expression of eHsp72 and eHsp27 during moderate- and high-intensity exercise may relate to the duration (i.e., core temperature attained) and intensity (i.e., rate of increase in core temperature) of exercise. Thus, the immuno-inflammatory release of eHsp72 and eHsp27 in response to exercise in the heat may be duration and intensity dependent.

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Acknowledgment

The authors thank all the subjects that participated in this investigation. The authors also thank Rhys Philips, Madeline Lynch, Colin Tuohy, Carl Cheah, Angelina Tan, and Thomas Wüthrich for their help with data collection. This work was supported by the University of Sydney Faculty of Health Sciences.

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Authors and Affiliations

  1. Research and Education Centre, ASPETAR, Qatar Orthopaedic and Sports Medicine Hospital, P.O. Box 29222, Doha, Qatar

    Julien D. Périard

  2. Discipline of Exercise and Sport Science, Faculty of Health Sciences, University of Sydney, Lidcombe, Australia

    Julien D. Périard, Patricia Ruell, Corinne Caillaud & Martin W. Thompson

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  1. Julien D. Périard
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  2. Patricia Ruell
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  3. Corinne Caillaud
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Correspondence to Julien D. Périard.

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Périard, J.D., Ruell, P., Caillaud, C. et al. Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity. Cell Stress and Chaperones 17, 375–383 (2012). https://doi.org/10.1007/s12192-011-0313-3

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  • DOI: https://doi.org/10.1007/s12192-011-0313-3

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