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Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography for the Determination of Trace Trichlorfon and Monocrotophos Residues in Fruits

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Abstract

In this study, a novel molecularly imprinted polymer (MIL@MIP) that could selectively recognize the trichlorfon and monocrotophos was synthesized using metal-organic framework MIL-101 as support material. The prepared MIL@MIP was characterized by Fourier transform infrared, thermogravimetric analysis, scanning electron microscope, static adsorption, kinetic adsorption, and competitive adsorption analyses. Using this material as sorbent, a new method of molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography for simultaneous determination of trichlorfon and monocrotophos was established. Under optimal conditions, the limit of detection (LOD) of this method for trichlorfon and monocrotophos detection was 0.011 and 0.015 mg/kg, respectively. The relative standard deviation (RSD) for five replicates of 0.05 mg/L trichlorfon and monocrotophos mixed solutions was within the range of 1.8 to 3.7 %. Apple and pear samples spiked with the two kinds of organophosphate pesticides were extracted and determined by using this method with good recoveries ranging from 86.5 to 91.7 %. Moreover, this method was successfully applied for the detection of the two organophosphate pesticides in strawberry samples.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (project no. 31471649 and 31171699) and Western Economic Uplift and Poverty Alleviation Development Projects of Shandong (project no. 49).

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Authors and Affiliations

  1. College of Food Science and Engineering, Shandong Agricultural University, Taian, 271018, China

    Deqing Li, Xin Zhang, Xuguang Qiao & Zhixiang Xu

  2. Shandong Wuzhou Detection Co., Ltd., Sishui, 273200, China

    Feifan Kong

Authors
  1. Deqing Li
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  2. Xin Zhang
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  3. Feifan Kong
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  4. Xuguang Qiao
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  5. Zhixiang Xu
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Corresponding authors

Correspondence to Xuguang Qiao or Zhixiang Xu.

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Funding

Dr. Zhixiang Xu has received research grants from the National Natural Science Foundation of China and Western Economic Uplift and Poverty Alleviation Development Projects of Shandong.

Conflict of Interest

Deqing Li declares that she has no conflict of interest. Xin Zhang declares that she has no conflict of interest. Feifan kong declares that he has no conflict of interest. Xuguang Qiao declares that he has no conflict of interest. Zhixiang Xu declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Xin Zhang and Deqing Li contributed equally to this work.

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Li, D., Zhang, X., Kong, F. et al. Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography for the Determination of Trace Trichlorfon and Monocrotophos Residues in Fruits. Food Anal. Methods 10, 1284–1292 (2017). https://doi.org/10.1007/s12161-016-0687-z

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  • DOI: https://doi.org/10.1007/s12161-016-0687-z

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