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Simultaneous Determination of Phenolic Compounds in Different Matrices using Phenyl-Hexyl Stationary Phase

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Abstract

A novel unique liquid chromatographic method has been developed consisting of diode array and fluorescence detection, using phenyl-hexyl stationary phase of the column, for the determination of over thirty phenolic compounds (anthocyanins, flavonols, flavan-3-ols, hydroxybenzoic acids, hydroxycinammic acids, and stilbenes). The method was validated including the following parameters: linearity, limits of detection and quantification, precision, and reproducibility. Excellent selectivity and sensitivity have been achieved. Detection levels ranging from 1.79 × 10−5 mg/L to 0.26 mg/L were obtained. New fluorescence wavelengths for detection of flavan-3-ols (λ ex = 225 and λ em = 320 nm) were more sensitive than previously published. The new optimized method showed good repeatability and reproducibility, and values for relative standard deviations (RSD) were less than 3 %. Applicability of the method was demonstrated for five different matrices, white and red wine from Vitis vinifera cultivars, apple cider, blackberry wine, and grape skin extracts, showing that method is robust and can be applied for routine analysis.

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References

  • Alonso E, Estrilla MI, Revilla E (1986) HPLC separation of flavonol glycosides in wines. Chromatographia 22:7–12

    Article  Google Scholar 

  • Baptiste JAB, da Tavares JFP, Carvalho RCB (2001) Comparison of polyphenols and aroma in red wines from Portuguese mainland Azores Islands. Food Res Int 34:345–355

    Article  Google Scholar 

  • Berente B, Reichenbächer M, Danzer K (2001) Improvement of the HPLC analysis of anthocyanins in red wines by use of recently developed columns. Fres J Anal Chem 371:68–72

    Article  CAS  Google Scholar 

  • Bonerz DPM, Pour Nikafardjam MS, Creasy GL (2008) A new RP-HPLC method for analysis of polyphenols and indole-3-acetic acid in wine. Am J Enol Vitic 59:106–109

    CAS  Google Scholar 

  • Chilla C, Guillen DA, Barroso CG, Perez-Bustamante JA (1996) Automated on line solid phase extraction-high performance liquid chromatography-diode array detection of phenolic compounds in sherry wine. J Chromatogr A 750:209–214

    Article  CAS  Google Scholar 

  • Del Almo M, Casado L, Hernandez V, Jimenez JJ (2004) Determination of free molecular phenolics and catechins in wine by solid phase extraction on polymeric cartridges and liquid chromatography with diode array detection. J Chromatogr A 1049:97–105

    Article  Google Scholar 

  • Dominguez C, Guillen DA, Barroso CG (2001) Automated solid-phase extraction for sample preparation followed by high-performance liquid chromatography with diode array and mass spectrometric detection for the analysis of resveratrol derivates in wine. J Chromatogr A 918:303–310

    Article  CAS  Google Scholar 

  • Gomez-Alonso S, Garcia-Romero E, Hermosin-Gutierrez I (2007) HPLC analysis of diverse grape and wine phenolics using direct injection and multidetection by DAD and fluorescence. J Food Comp Anal 20:618–626

    Article  CAS  Google Scholar 

  • Jeandet P, Brenil AC, Adrian M, Weston LA, Debord S, Meunier P, Maume G, Bessis R (1997) HPLC analysis of grapevine phytoalexins coupling photodiode array detection and fluorometry. Anal Chem 69:5172–5177

    Article  CAS  Google Scholar 

  • Jeffery DW, Mercurio MD, Herderich MJ, Hayasaka Y, Smith PA (2008) Rapid isolation of red wine polymeric polyphenols by solid phase extraction. J Agric Food Chem 56:2571–2580

    Article  CAS  Google Scholar 

  • Katalinić V, Miloš M, Modun D, Boban M (2004) Antioxidant effectiveness of selected wines in comparison with (+)-catechin. Food Chem 86:593–600

    Article  Google Scholar 

  • Komes D, Urlich D, Kovačević Ganić K, Lovrić T (2007) Study of phenols and volatile composition of white wine during fermentation and a short time of storage. Vitis 46:77–84

    Google Scholar 

  • Lamuela-Raventos RM, Waterhouse AL (1994) A direct HPLC separation of wine phenolics. Am J Enol Vitic 45:1–5

    CAS  Google Scholar 

  • Matejiček D, Klejdus B, Mikeš O, Šterbova D, Kuban V (2003) Application of solid phase extraction for determination of phenolic compounds in barrique wines. Anal Bioanal Chem 377:340–345

    Article  Google Scholar 

  • Merken HM, Beecher GR (2000) Measurement of food flavonoids by high performance liquid chromatography: a review. J Agric Food Chem 48:577–599

    Article  CAS  Google Scholar 

  • Monagas M, Bartolome B, Gomez-Cordoves C (2005a) Updated knowledge about the presence of phenolic compounds in wine. Crit Rev Food Sci Nutr 45:85–118

    Article  CAS  Google Scholar 

  • Monagas M, Suarez R, Gomez-Cordoves C, Bartolome B (2005b) Simultaneous determination of non-anthocyanin phenolic compound in red wines by HPLC-DAD/ESI-MS. Am J Enol Vitic 56:139–147

    CAS  Google Scholar 

  • Negishi O, Ozawa T (1996) Determination of hydroxycinammic acids, hydroxybenzoic acids, hydroxylbenzaldehydes, hydroxybenzyl alcohols and their glucosides by high performance liquid chromatography. J Chromatogr A 756:129–136

    Article  Google Scholar 

  • Perez-Magarino S, Ortega-Heras M, Cano-Mozo E (2008) Optimization of a solid-phase extraction method using copolymer sorbents for isolation of phenolic compounds in red wines and quantification by HPLC. J Agric Food Chem 56:11560–11570

    Article  CAS  Google Scholar 

  • Pinelo M, Laurie VF, Waterhouse AL (2006) A simple method to separate red wine nonpolymeric and polymeric phenols by solid phase extraction. J Agric Food Chem 54:2839–2844

    Article  CAS  Google Scholar 

  • Plavša T, Jurinjak N, Antunović D, Peršurić Đ, Ganić Kovačević K (2012) The influence of skin maceration time on the phenolic composition and antioxidant activity of red wine Teran (Vitis vinifera L.). Food Technol Biotechnol 50:152–158

    Google Scholar 

  • Rastija V, Srečnik G, Medić-Šarić M (2009) Polyphenolic composition of Croatian wines with different geographical origins. Food Chem 115:54–60

    Article  CAS  Google Scholar 

  • Rodriguez Delgado MA, Malovana S, Perez JP, Borges T, Garcia Montelongo FJ (2001) Separation of phenolic compounds by high performance liquid chromatography with absorbance and fluorimetric detection. J Chromatogr A 912:249–257

    Article  CAS  Google Scholar 

  • Sun B, Ribes AM, Leandro M, Belchior AP, Spranger MI (2006a) Stilbenes: quantitative extraction from grape skins, contribution of grape solids to wine and variation during wine maturation. Anal Chim Acta 563:382–390

    Article  CAS  Google Scholar 

  • Sun B, Leandro MC, De Freitas V, Spranger MI (2006b) Fractionation of red wine polyphenols by solid phase extraction and liquid chromatography. J Chromatogr A 1128:27–38

    Article  CAS  Google Scholar 

  • Terrier N, Poncet-Legrand C, Cheynier V (2009) Flavanols, flavonols and dihydroflavonols. In: Polo MC, Moreno-Arribas MV (eds) Wine chemistry and biochemistry. Springer, New York, pp 463–507

    Chapter  Google Scholar 

  • Vitrac X, Monti JP, Vercauteren J, Defieux G, Merillon JM (2002) Direct liquid chromatographic analysis of resveratrol derivates and flavanonols in wines with absorbance and fluorescence detection. Anal Chim Acta 458:103–110

    Article  CAS  Google Scholar 

  • Zotou A, Frangi E (2008) Development and validation of an SPE-LC method for the simultaneous determination of trans-resveratrol and selected flavonoids in wine. Chromatographia 67:789–793

    Article  CAS  Google Scholar 

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Conflict of Interest

Ivana Tomaz declares that she has no conflict of interest. Luna Maslov declares that she has no conflict of interest.

Compliance with Ethics Requirements

This is an original research article that has neither been published previously nor considered presently for publication elsewhere. All authors named in a manuscript are entitled to the authorship and have approved the final version of the submitted manuscript. This article does not contain any studies with human or animal subjects.

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    1. Department of Viticulture and Enology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, Zagreb, Croatia

      Ivana Tomaz & Luna Maslov

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    1. Ivana Tomaz
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    2. Luna Maslov
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    Correspondence to Luna Maslov.

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    Ivana Tomaz and Luna Maslov contributed equally to this work.

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    Tomaz, I., Maslov, L. Simultaneous Determination of Phenolic Compounds in Different Matrices using Phenyl-Hexyl Stationary Phase. Food Anal. Methods 9, 401–410 (2016). https://doi.org/10.1007/s12161-015-0206-7

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