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Simultaneous Determination of Three Trace Organophosphorus Pesticide Residues in Vegetables Using Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography

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Abstract

In this study, a molecularly imprinted polymer that can selectively recognize dimethoate, isocarbophos, and methyl parathion was synthesized. Using this compound as sorbent, a new method for simultaneous determination of the three organophosphorus pesticides by molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography was established. The factors affecting the preconcentration and sensitivity were studied in detail. Under optimal conditions, the limits of detection of this method for dimethoate, isocarbophos, and methyl parathion were 19.78, 8.73, and 17.41 μg/kg, respectively. The relative standard deviation for five replicates of 0.01 mg/L-mixed solutions was in the range of 1.8–4.2 %. Cucumber sample spiked with the three organophosphorus pesticides at levels of 0.125 and 0.250 μg/g were examined using this method, good recoveries were obtained ranging from 82.5 to 95.6 %. Moreover, this method was also successfully used for the detection of the three organophosphorus pesticides in cauliflower sample.

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Acknowledgments

The authors are grateful for the financial support from the National Natural Science Foundation of China (Project No. 31171699 and 31471649) and the Agricultural Science and Technology Achievements Transformation Project of China (Project No. 2013GB2C600276).

Compliance with Ethics Requirements

We have no financial relationship with the organization that sponsored the research. Dr. Zhixiang Xu has received research grants from the National Natural Science Foundation of China and the Agricultural Science and Technology Achievements Transformation Project of China.

Conflict of Interest

Qingqing Wang, Xin Zhang, Zhixiang Xu and Huiju Gao declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects.

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    Authors and Affiliations

    1. College of Food Science and Engineering, Shandong Agricultural University, Taian, 271018, China

      Qingqing Wang, Xin Zhang & Zhixiang Xu

    2. College of Forestry, Shandong Agricultural University, Taian, 271018, China

      Huiju Gao

    Authors
    1. Qingqing Wang
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    2. Xin Zhang
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    3. Zhixiang Xu
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    4. Huiju Gao
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    Corresponding author

    Correspondence to Huiju Gao.

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    The authors Xin Zhang and Qingqing Wang contributed equally to this work.

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    Wang, Q., Zhang, X., Xu, Z. et al. Simultaneous Determination of Three Trace Organophosphorus Pesticide Residues in Vegetables Using Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography. Food Anal. Methods 8, 2044–2051 (2015). https://doi.org/10.1007/s12161-014-0086-2

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    • DOI: https://doi.org/10.1007/s12161-014-0086-2

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