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A Rapid Quantification of β-Carotene in Fruits and Vegetables by Dispersive Liquid–Liquid Microextraction Coupled with UV–Vis Spectrophotometry: Optimized by Response Surface Methodology

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Abstract

A simple, fast, and efficient method consisted of optimized dispersive liquid–liquid microextraction (DLLME) followed by UV–vis spectrophotometry was developed for determination of β-carotene in fruits and vegetables. Chloroform and methanol were chosen as extraction and disperser solvents, respectively. The extraction process was optimized using a central composite design (CCD) with the optimum points of 115 μL for volume of extraction solvent and 6.5 % (w/v) for salt concentration. Under the optimal conditions, the relative standard deviation (RSD, C = 500 μg L−1, n = 5), limit of detection (LOD), linear dynamic range (LDR), and coefficient of determination (R2) were 1.08 %, 2 μg L−1, 50–1,500 μg L−1, and 0.991, respectively. The present method consisted of a simple and fast sample preparation procedure without any antioxidant addition, saponification, and purification was used.

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Conflict of Interest

Hassan Sereshti declares that he has no conflict of interest. Mohammad Ahmadvand declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, University of Tehran, P.O. Box 14155-64555, Tehran, Iran

    Hassan Sereshti, Mohammad Ahmadvand & Shohreh Asgari

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  1. Hassan Sereshti
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  2. Mohammad Ahmadvand
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  3. Shohreh Asgari
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Correspondence to Hassan Sereshti.

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Sereshti, H., Ahmadvand, M. & Asgari, S. A Rapid Quantification of β-Carotene in Fruits and Vegetables by Dispersive Liquid–Liquid Microextraction Coupled with UV–Vis Spectrophotometry: Optimized by Response Surface Methodology. Food Anal. Methods 7, 1481–1488 (2014). https://doi.org/10.1007/s12161-013-9777-3

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  • DOI: https://doi.org/10.1007/s12161-013-9777-3

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