Skip to main content
SpringerLink
Log in

Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)

  • Technical Note
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Our aim was to assess the suitability of ultra-high performance liquid chromatography (UHPLC) for the simultaneous determination of biomarkers of vitamins A (retinol, retinyl esters), E (α- and γ-tocopherol), D (25-OH-vitamin D), and the major carotenoids in human serum to be used in clinical practice. UHPLC analysis was performed on HSS T3 column (2.1 × 100 mm; 1.8 μm) using gradient elution and UV–VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, α- and γ-tocopherol, lutein plus zeaxanthin, α-carotene, β-carotene, α- and β-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Fidanza F (1994) Nutritional status assessment in perspective. Bibl Nutr Dieta 51:9–18

    Google Scholar 

  2. Van den Berg H (1994) Functional vitamin status assessment. Bibl Nutr Dieta 51:142–149

    Google Scholar 

  3. Aggett PJ, Antoine JM, Asp NG, Contor L, Cummings JH, Howlett J (2005) PASSCLAIM. Process for the assessment of scientific support for claims on foods. Eur J Nutr (suppl 1) 44:1–30

    Google Scholar 

  4. Drenowski A, Maillot M, Darmon N (2009) Testing nutrient profile models in relation to energy density and energy cost. Eur J Nutr 63(5):674–683

    Article  Google Scholar 

  5. Citová I, Havlíková L, Urbánek L, Solichová D, Nováková L, Solich P (2007) Comparison of a novel ultra-performance liquid chromatographic method for determination of retinol and a-tocopherol in human serum with conventional HPLC using monolithic and particulate columns. Anal Bioanal Chem 388:675–681

    Article  Google Scholar 

  6. Paliakov EM, Crow BS, Bishop MJ, Norton D, George J, Bralley JA (2009) Rapid quantitative determination of fat-soluble vitamins and coenzyme Q10 in humna serum by reversed phase ultra-performance liquid chromatography with UV detection. J Chromatogr B Analyt Technol Biomed Life Sci. 1;877(1–2):89–94.

    Google Scholar 

  7. Olmedilla B, Granado F, Gil-Martínez E, Rojas-Hidalgo E (1997) Reference levels of retinol, a-tocopherol and main carotenoids in serum of control and insulin-dependent diabetic Spanish subjects. Clin Chem 43(6):1066–1071

    CAS  Google Scholar 

  8. Granado-Lorencio F, Olmedilla-Alonso B, Herrero-Barbudo C, Blanco-Navarro I, Blázquez-García S, Pérez-Sacristán B (2006) Simultaneous determination of vitamins A, E and 25-OH-vitamin D application in clinical assessment. Clin Biochem 39(2):180–182

    Article  CAS  Google Scholar 

  9. German JB, Watkins SM (2004) Metabolic assessment; a key to nutritional strategies for health. Trends Food Sci Technol 15(54):1549

    Google Scholar 

  10. Van Ommen B, Fairweather-Tait S, Freidig A, Kardinaal A, Sclabert A, Wopereis S (2008) A network biology model of micronutrient related health. Brit J Nutr 99(suppl 3):S72–S80

    Google Scholar 

  11. Binkley N, Krueger D, Cowgill CS, Plum L, Lake E, Hansen KE, De luca HF, Drezner MK (2004) Assay variation confounds the diagnosis of hypovitaminosis D: A call for standardization. J Clin Endocrinol Metab 89(7):3152–3157

    Article  CAS  Google Scholar 

  12. Leino A, Turpeinen U, Koskinen P (2008) Automated measurement of 25-OH-vitamin D3 on the Roche modular E170 analyzer. Clin Chem 54(12):2059–2062

    Article  CAS  Google Scholar 

  13. Granado-Lorencio F, Salazar Mosteiro J, Herrero-Barbudo C, Donoso Navarro E, Blanco-Navarro I, Pérez-Sacristán B (2009) 25-OH-Vitamin D assay variation and subject management in clinical practice. Clin Biochem. doi:10.1016/j.clinbiochem.2009.11.020

    Google Scholar 

  14. Knox S, Harris J, Calton L, Wallace AM (2009) A simple automated solid-phase extraction procedure for measurement of 25-hydroxyvitamin D3 and D2 by liquid chromatography–tandem mass spectrometry. Annals Clin Biochem 46:226–230

    Article  CAS  Google Scholar 

  15. Aronov PA, Hall LM, Dettmer K, Stephensen CB, Hammock BD (2008) Metabolic profiling of major vitamin D metabolites using Diels–Alder derivatization and ultra-performance liquid chromatography–tandem mass spectrometry. Anal Bioanal Chem 391:1917–1930

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unidad de Vitaminas. Bioquímica Clínica, Edificio laboratorios (Peine 7) Planta 1ª, Hospital Universitario Puerta de Hierro-Majadahonda, c/ Maestro Joaquin Rodrigo, 2, 28222, Madrid, Spain

    F Granado-Lorencio, C Herrero-Barbudo, I Blanco-Navarro & B Pérez-Sacristán

Authors
  1. F Granado-Lorencio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C Herrero-Barbudo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I Blanco-Navarro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B Pérez-Sacristán
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to F Granado-Lorencio.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Granado-Lorencio, F., Herrero-Barbudo, C., Blanco-Navarro, I. et al. Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids). Anal Bioanal Chem 397, 1389–1393 (2010). https://doi.org/10.1007/s00216-010-3655-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-010-3655-2

Keywords

Search

Navigation