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A Halophilic, Alkalithermostable, Ionic Liquid-Tolerant Cellulase and Its Application in In Situ Saccharification of Rice Straw

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Abstract

A cellulase, En5H, from halophilic, alkalithermophilic Alkalilimnicola sp. NM-DCM1 was expressed and purified. En5H had maximal hydrolytic activity at 55 °C, pH 8.8, and 2.5 M NaCl. En5H is β-1,4 linkage-specific, hydrolyzing carboxymethyl cellulose, Avicel, cellobiose, and p-nitrophenyl β-d-glucopyranoside. En5H was resistant to inhibitors and organic solvents. The half-life of En5H was increased 16–43-fold when incubated in 20% (v/v) of ionic liquids (IL) at 55 °C in the presence of 2.5 M NaCl, and maximal hydrolytic activity of En5H in 10% (v/v) 1-allyl-3-methylimidazolium chloride and 1,3-dimethylimidazolium dimethyl phosphate was 122 and 110%, respectively, as compared with activity in buffer. A cellulase-IL system combining IL pretreatment and enzymatic saccharification was tested. With an enzyme load of 110 U/g rice straw, the conversion of rice straw cellulose and hemicellulose increased by 28% compared with unpretreated rice straw. En5H has potential for use in transformation of lignocellulose to glucose in a single-step process.

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Acknowledgements

This work was supported in part by the US-Egypt Science and Technology Joint Fund in cooperation with the Suez Canal University (Egypt) under project number 1841 and the University of Georgia (USA) under project number NSF-OISE-1132412.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt

    Noha M. Mesbah

  2. Department of Microbiology, University of Georgia, Athens, GA, 30602, USA

    Juergen Wiegel

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  1. Noha M. Mesbah
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  2. Juergen Wiegel
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Correspondence to Noha M. Mesbah.

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Mesbah, N.M., Wiegel, J. A Halophilic, Alkalithermostable, Ionic Liquid-Tolerant Cellulase and Its Application in In Situ Saccharification of Rice Straw. Bioenerg. Res. 10, 583–591 (2017). https://doi.org/10.1007/s12155-017-9825-8

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