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Matrix assisted laser desorption ionisation ion mobility separation mass spectrometry imaging of ex-vivo human skin

  • Original Research
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International Journal for Ion Mobility Spectrometry

Abstract

Matrix assisted laser desorption ionisation ion mobility separation mass spectrometry imaging (MALDI-IMS-MSI) has been employed to image the distribution of proteins in ex-vivo human skin. Using a “bottom-up” proteomics approach employing “on-tissue” digestion the distribution of abundant skin proteins; collagen, keratin, decorin and serum albumin could be mapped. Images have been recorded at 150 and 30 μm spatial resolution. Multivariate statistical analysis of the data has been employed to associate specific proteins with layers of the skin. The improved specificity given by the use of ion mobility separation in mass spectrometric imaging has been demonstrated by separation of peptide ions from phospholipids.

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Authors and Affiliations

  1. Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield, S11WB, UK

    Philippa J. Hart, Simona Francese, M. Nicola Woodroofe & Malcolm R. Clench

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  1. Philippa J. Hart
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  2. Simona Francese
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  3. M. Nicola Woodroofe
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  4. Malcolm R. Clench
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Correspondence to Malcolm R. Clench.

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Hart, P.J., Francese, S., Woodroofe, M.N. et al. Matrix assisted laser desorption ionisation ion mobility separation mass spectrometry imaging of ex-vivo human skin. Int. J. Ion Mobil. Spec. 16, 71–83 (2013). https://doi.org/10.1007/s12127-013-0124-6

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  • DOI: https://doi.org/10.1007/s12127-013-0124-6

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