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Sequence-specific 1H, 15N, and 13C resonance assignments of the autophagy-related protein LC3C

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Abstract

Autophagy is a versatile catabolic pathway for lysosomal degradation of cytoplasmic material. While the phenomenological and molecular characteristics of autophagic non-selective (bulk) decomposition have been investigated for decades, the focus of interest is increasingly shifting towards the selective mechanisms of autophagy. Both, selective as well as bulk autophagy critically depend on ubiquitin-like modifiers belonging to the Atg8 (autophagy-related 8) protein family. During evolution, Atg8 has diversified into eight different human genes. While all human homologues participate in the formation of autophagosomal membrane compartments, microtubule-associated protein light chain 3C (LC3C) additionally plays a unique role in selective autophagic clearance of intracellular pathogens (xenophagy), which relies on specific protein–protein recognition events mediated by conserved motifs. The sequence-specific 1H, 15N, and 13C resonance assignments presented here form the stepping stone to investigate the high-resolution structure and dynamics of LC3C and to delineate LC3C’s complex network of molecular interactions with the autophagic machinery by NMR spectroscopy.

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Authors and Affiliations

  1. Institute of Complex Systems, ICS-6: Structural Biochemistry, Forschungszentrum Jülich, 52425, Jülich, Germany

    Carsten Krichel, Oliver H. Weiergräber, Marina Pavlidou, Jeannine Mohrlüder, Melanie Schwarten, Dieter Willbold & Philipp Neudecker

  2. Institut für Physikalische Biologie und BMFZ, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany

    Carsten Krichel, Dieter Willbold & Philipp Neudecker

Authors
  1. Carsten Krichel
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  2. Oliver H. Weiergräber
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  3. Marina Pavlidou
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  4. Jeannine Mohrlüder
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  5. Melanie Schwarten
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  6. Dieter Willbold
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  7. Philipp Neudecker
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Correspondence to Philipp Neudecker.

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Krichel, C., Weiergräber, O.H., Pavlidou, M. et al. Sequence-specific 1H, 15N, and 13C resonance assignments of the autophagy-related protein LC3C. Biomol NMR Assign 10, 41–43 (2016). https://doi.org/10.1007/s12104-015-9633-z

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  • DOI: https://doi.org/10.1007/s12104-015-9633-z

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