Skip to main content
SpringerLink
Log in

Backbone NMR assignments of DFP-inhibited mature subtilisin E

  • Article
  • Published:
Biomolecular NMR Assignments Aims and scope Submit manuscript

Abstract

Here we report the backbone chemical shifts of the DFP-inhibited mature subtilisin E, which was uniformly labeled by 13C, 15N with a supplement of excess calcium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bryan P, Wang L, Hoskins J, Ruvinov S, Strausberg S, Alexander P, et al (1995) Catalysis of a protein folding reaction: mechanistic implications of the 2.0 a structure of the subtilisin-prodomain complex. Biochemistry 34:10310–10318. doi:10.1021/bi00032a026

    Article  Google Scholar 

  • Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293. doi:10.1007/BF00197809

    Article  Google Scholar 

  • Ferentz AE, Wagner G (2000) NMR spectroscopy: a multifaceted approach to macromolecular structure. Q Rev Biophys 33:29–65. doi:10.1017/S0033583500003589

    Article  Google Scholar 

  • Ikemura H, Inouye M (1988) In vitro processing of pro-subtilisin produced in Escherichia coli. J Biol Chem 263:12959–12963

    Google Scholar 

  • Ikemura H, Takagi H, Inouye M (1987) Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. J Biol Chem 262:7859–7864

    Google Scholar 

  • Inouye M (1991) Intramolecular chaperone: the role of the pro-peptide in protein folding. Enzyme 45:314–321

    Google Scholar 

  • Keller R (2004) The computer aided resonance assignment tutorial. Cantina Verlag, Switzerland

    Google Scholar 

  • Marsh JA, Singh VK, Jia Z, Forman-Kay JD (2006) Sensitivity of secondary structure propensities to sequence differences between alpha- and gamma-synuclein: implications for fibrillation. Protein Sci 15:2795–2804. doi:10.1110/ps.062465306

    Article  Google Scholar 

  • Sari N, Fisher KE, Bryan PN, Orban J (2007) Main chain NMR assignments of subtilisin Sbt70 in its prodomain-bound state. Biomol NMR Assign 1:209–211

    Article  Google Scholar 

  • Sattler M, Schleucher JG, Griesinger C (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog NMR Spectrosc 34:93–158. doi:10.1016/S0079-6565(98)00025-9

    Article  Google Scholar 

  • Yabuta Y, Subbian E, Takagi H, Shinde U, Inouye M (2002) Folding pathway mediated by an intramolecular chaperone: dissecting conformational changes coincident with autoprocessing and the role of Ca(2+) in subtilisin maturation. J Biochem 131:31–37

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Robert-Wood-Johnson Medical School, 675 Hoes Ln, Piscataway, NJ, 08854, USA

    Yu-Jen Chen, Liliana Falzon & Masayori Inouye

  2. Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Rd, Piscataway, NJ, 08854, USA

    Kuen-Phon Wu, Seho Kim & Jean Baum

  3. BioMaPS Institute for Quantitative Biology, Rutgers University, 610 Taylor Rd, Piscataway, NJ, 08854, USA

    Jean Baum

Authors
  1. Yu-Jen Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kuen-Phon Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Seho Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liliana Falzon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masayori Inouye
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jean Baum
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Masayori Inouye or Jean Baum.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, YJ., Wu, KP., Kim, S. et al. Backbone NMR assignments of DFP-inhibited mature subtilisin E. Biomol NMR Assign 2, 131–133 (2008). https://doi.org/10.1007/s12104-008-9103-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12104-008-9103-y

Keywords

Search

Navigation