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Clinical significance of expression level of CX3CL1–CX3CR1 axis in bone metastasis of lung cancer

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Abstract

Purpose

To investigate the clinical significance of CX3 chemokine ligand 1(CX3CL1) and CX3CR1 in patients with bone metastasis from lung cancer. The expression levels of CX3CL1 and CX3CR1 mRNA and protein in primary lung cancer and lung cancer bone metastasis were detected by qRT-PCR and Western blot.

Methods

One hundred patients with lung cancer were divided into a boneless metastasis group (50 patients with bone metastasis) and a bone metastasis group (50 patients without distant metastasis). The bone transfer component was graded by Soloway classification (0 to III). The expression levels of serum CX3CL1–CX3CR1 axis were detected by enzyme-linked immunosorbent assay (ELISA). RT-qPCR and Western Blot were used to verify the transfection efficiency. The scratching assay was used to detect the migration of CX3CL1 to 95-D cells after down-regulating the expression of CX3CR1.

Results

The expression levels of CX3CL1 and CX3CR1 mRNA and protein in the primary lung cancer and lung cancer bone metastasis were significantly higher than those in the adjacent tissues (P < 0.0001). The levels of serum CX3CL1 and CX3CR1 in bone metastasis group were significantly higher than those in boneless metastasis group and healthy control group (P < 0.05). In the bone metastasis group, the levels of serum CX3CL1 and CX3CR1 were significantly positively correlated with the degree of disease progression (P < 0.01).

Conclusion

The expression level of serum CX3CL1–CX3CR1 axis is expected to be an auxiliary reference index for monitoring bone metastasis of lung cancer.

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Authors and Affiliations

  1. Test Room of Clinical Laboratory, The First Affiliated Hospital of Harbin Medical University, 199 Dongdazhi Street, Nangang District, Harbin, 150001, Heilongjiang, China

    Y. Liu

  2. Department of Human Resources, Xiamen Hospital of T.C.M, Xiamen, 361000, China

    H. Ma

  3. Department of Obstetrics, Xiamen Hospital of T.C.M, Xiamen, 361000, China

    T. Dong

  4. Department of Clinical Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China

    Y. Yan

  5. Ward 1, Department of Thoracic Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China

    L. Sun

  6. Department of Clinical Laboratory, The Second Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, 150001, China

    W. Wang

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  1. Y. Liu
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  2. H. Ma
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  3. T. Dong
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  4. Y. Yan
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  5. L. Sun
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  6. W. Wang
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Contributions

YL, HM and TD are responsible for the conception or design of the work. YL, YY, LS and WW contribute the acquisition, analysis, or interpretation of data for the work. HM and YY provide the tissue samples. TD helps in the follow-up of the patients. LS helps in reviewing the histopathology slides. All authors finally approved the manuscript version to be published. YL is the guarantor of the article.

Corresponding author

Correspondence to Y. Liu.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval (Research involving human participants and/or animals)

The study was approved by Ethical Committee of The First Affiliated Hospital of Harbin Medical University and conducted in accordance with the ethical standards.

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All study subjects signed informed consent prior to their participation.

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Liu, Y., Ma, H., Dong, T. et al. Clinical significance of expression level of CX3CL1–CX3CR1 axis in bone metastasis of lung cancer. Clin Transl Oncol 23, 378–388 (2021). https://doi.org/10.1007/s12094-020-02431-6

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  • DOI: https://doi.org/10.1007/s12094-020-02431-6

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