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Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis

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Abstract

Objectives

MicroRNA-200 family (miR-200f) has been consistently reported to be deregulated and modulate the metastatic process in multiple cancers. In the present study, we detected the expression of miR-200f in breast cancer (BC) tissue and explored its relationships with clinicopathological characteristics, especially with lymph node metastasis.

Methods

Expression levels of miR-200a, miR-200b, miR-200c, miR-141, and miR-429 in 99 pairs of BC tissues and adjacent normal tissues were measured by real-time quantitative PCR. The correlation between miR-200f level and multiple clinicopathological factors was then examined by Mann–Whitney test, ANOVA, and operating characteristic (ROC) analysis.

Results

All members of the miR-200f were down-regulated in BC tissue compared with that in normal adjacent tissue; miR-200a, miR-200b, and miR-200c were highly decreased (p < 0.05), while the differences of miR-141 and miR-429 between patients and the control group were not statistically significant. Furthermore, all five members were found to be distinctly decreased with the incidence of lymph node metastasis (p < 0.05); When the patients were divided into three groups according to the number of lymph node metastasis (0; 1–3; ≥4), a gradual decrease of miR-200f expression was observed with the increasing number of lymph node metastasis; ROC revealed that miR-200b can differentiate patients with lymph node metastasis from those without lymph node metastasis.

Conclusion

These observations imply that the down-regulation of miR-200f in human BC is associated with an invasive phenotype, and miR-200b may be useful to estimate the likelihood of the presence of pathologically positive lymph nodes.

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Acknowledgments

This work was supported in part by the National Natural Science Foundation of China (Grant Number: 81172513, 81071028).

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    Authors and Affiliations

    1. The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha, 410078, Hunan, China

      Hua He, Wen Huang, Yunting Lin, Shiyu Luo, Qian Du & Ranhui Duan

    2. Department of Breast and Thyroid Surgery, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China

      Feng Xu

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    1. Feng Xu
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    2. Hua He
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    3. Wen Huang
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    7. Ranhui Duan
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    Correspondence to Ranhui Duan.

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    The authors declare no conflict of interest.

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    Feng Xu and Hua He contributed equally to this article.

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    Xu, F., He, H., Huang, W. et al. Decreased expression of MicroRNA-200 family in human breast cancer is associated with lymph node metastasis. Clin Transl Oncol 18, 283–288 (2016). https://doi.org/10.1007/s12094-015-1364-1

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    • DOI: https://doi.org/10.1007/s12094-015-1364-1

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