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Efficient Using Durian Shell Hydrolysate as Low-Cost Substrate for Bacterial Cellulose Production by Gluconacetobacter xylinus

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Abstract

Durian is one important tropical fruit with high nutritional value, but its shell is usually useless and considered as waste. To explore the efficient and high-value utilization of this agricultural and food waste, in this study, durian shell was simply hydrolyzed by dilute sulfuric acid, and the durian shell hydrolysate after detoxification was used for bacterial cellulose (BC) production by Gluconacetobacter xylinus for the first time. BC was synthesized in static culture for 10 days and the highest BC yield (2.67 g/L) was obtained at the 8th day. The typical carbon sources in the substrate including glucose, xylose, formic acid, acetic acid, etc. can be utilized by G. xylinus. The highest chemical oxygen demand (COD) removal (16.40%) was obtained at the 8th day. The highest BC yield on COD consumption and the highest BC yield on sugar consumption were 93.51% and 22.98% (w/w), respectively, suggesting this is one efficient bioconversion for BC production. Durian shell hydrolysate showed small influence on the BC structure by comparison with the structure of BC generated in traditional Hestrin–Schramm medium detected by FE-SEM, FTIR, and XRD. Overall, this technology can both solve the issue of waste durian shell and produce valuable bio-polymer (BC).

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Acknowledgements

The authors acknowledge the financial support of the Science and Technology Project of Guangdong Province (2016A010105016), National Natural Science Foundation of China (21606229, 51378486, 31600475), project of Guangzhou Science and Technology (201610010014), Project of Huai-An Science and Technology (HAS2015035), Youth Innovation Promotion Association CAS (2015290), and Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences (y407r41001), Key Laboratory of Renewable Energy, Chinese Academy of Sciences (Y707j41001).

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Authors and Affiliations

  1. Key Laboratory of Renewable Energy, Chinese Academy of Sciences, No. 2 Nengyuan Road, Tianhe District, Guangzhou, 510640, People’s Republic of China

    Mu-Tan Luo, Cheng Zhao, Chao Huang, Xue-Fang Chen, Qian-Lin Huang, Gao-Xiang Qi, Lan-Lan Tian, Lian Xiong, Hai-Long Li & Xin-De Chen

  2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People’s Republic of China

    Mu-Tan Luo, Cheng Zhao, Chao Huang, Xue-Fang Chen, Qian-Lin Huang, Gao-Xiang Qi, Lan-Lan Tian, Lian Xiong, Hai-Long Li & Xin-De Chen

  3. Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou, 510640, People’s Republic of China

    Mu-Tan Luo, Cheng Zhao, Chao Huang, Xue-Fang Chen, Qian-Lin Huang, Gao-Xiang Qi, Lan-Lan Tian, Lian Xiong, Hai-Long Li & Xin-De Chen

  4. Xuyi Center of Attapulgite Research Development and Industrialization, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi, 211700, People’s Republic of China

    Xin-De Chen

  5. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Mu-Tan Luo, Cheng Zhao, Qian-Lin Huang, Gao-Xiang Qi & Lan-Lan Tian

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  1. Mu-Tan Luo
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Luo, MT., Zhao, C., Huang, C. et al. Efficient Using Durian Shell Hydrolysate as Low-Cost Substrate for Bacterial Cellulose Production by Gluconacetobacter xylinus . Indian J Microbiol 57, 393–399 (2017). https://doi.org/10.1007/s12088-017-0681-1

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