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Identification of Genes Differentially Expressed Between Ochratoxin-Producing and Non-Producing Strains of Aspergillus westerdijkiae

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Abstract

Approximately 70 % of Aspergillus westerdijkiae strains are able to produce ochratoxin A (OTA), a nephrotoxic and carcinogenic mycotoxin which have been found in cereal and food commodities. Despite of its importance there is, up to now, no information available about which genes are differentially expressed between A. westerdijkiae ochratoxin-producing and non-producing strains. Using cDNA RDA approach we successfully sequenced 231 raw ESTs expected to be enriched in the ochratoxin-producing strain. BLASTX searches against the public databases showed that of these, 205 ESTs (79 %) exhibited significant similarities with proteins of known functions, 28 ESTs (11 %) had matches to hypothetical proteins, and the remaining 27 ESTs (10 %) had no significant hits. EST alignment resulted in a total of 14 non-redundant consensus sequences. Three putative genes encoding oxidoreductases were validated as up-expressed in the OTA producer strain using RT-qPCR approach. The expression of the putative genes encoding a cytochrome P450 family protein, 3-hydroxyphenylacetate-6-hydroxylase, and endoplasmic reticulum oxidoreductin were higher (32-, 2.8- and 20-fold respectively) in the OTA producer strain compared to the non-producer strain.

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Acknowledgments

This work was supported by Grants and fellowships from the Brazilian institutions, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Araucária.

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Authors and Affiliations

  1. Universidade Estadual de Londrina, P.O. Box 6001, Londrina, 86051-990, Brazil

    Daniele Sartori, Fernanda Pelisson Massi, Larissa Souza Ferranti & Maria Helena P. Fungaro

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  1. Daniele Sartori
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  2. Fernanda Pelisson Massi
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  3. Larissa Souza Ferranti
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  4. Maria Helena P. Fungaro
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Corresponding author

Correspondence to Maria Helena P. Fungaro.

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Sartori, D., Massi, F.P., Ferranti, L.S. et al. Identification of Genes Differentially Expressed Between Ochratoxin-Producing and Non-Producing Strains of Aspergillus westerdijkiae . Indian J Microbiol 54, 41–45 (2014). https://doi.org/10.1007/s12088-013-0408-x

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  • DOI: https://doi.org/10.1007/s12088-013-0408-x

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