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First Report of OXA-4, an ESBL Isolated from Pseudomonas aeruginosa a South Indian Strain

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Abstract

The OXA-type β-lactamases are so named because of their oxacillin-hydrolyzing abilities. In this study we characterize an extended spectrum β-lactamase, designated OXA-4, produced by a clinical isolate of Pseudomonas aeruginosa. ESBL production was detected by double disk synergy test. The P. aeruginosa isolate was obtained from endotracheal suction tip of 84 years old male patient diagnosed with CVA and hypertension. ESBL producing OXA β-lactamases was detected by PCR with primers specific to the conserved regions of the coding genes. Iso electric focusing was done to confirm the significance, sequencing the amplified product was also done. In the phenotypic identification, the strain was highly resistant to third generation cephalosporins and also to imipenem. The PCR amplified product for OXA β-lactamase was viewed at 919 bp. The pI point for the same was identified at 7.2. With the help of sequencing the amplified OXA β-lactamase was identified as OXA-4 gene. Here we report P. aeruginosa producing OXA-4 ESBL for the first time in the Indian subcontinent.

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  1. S. A. Jemima Kingsley

    Present address: , # 17A, Rajarathinam 4th Street, New Narmada Nagar, Ullagaram, Chennai, 600 091, Tamilnadu, India

Authors and Affiliations

  1. Department of Microbiology, International Centre for Cardio Thoracic and Vascular Diseases, A Unit of Frontier Lifeline, Chennai, 600 101, Tamilnadu, India

    S. A. Jemima Kingsley & Susan Verghese

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  2. Susan Verghese
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Correspondence to S. A. Jemima Kingsley.

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Kingsley, S.A.J., Verghese, S. First Report of OXA-4, an ESBL Isolated from Pseudomonas aeruginosa a South Indian Strain. Indian J Microbiol 53, 308–314 (2013). https://doi.org/10.1007/s12088-012-0326-3

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  • DOI: https://doi.org/10.1007/s12088-012-0326-3

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