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Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System

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Abstract

Metabolic activities of four Bacillus strains to transform glucose into hydrogen (H2) and polyhydroxybutyrate (PHB) in two stages were investigated in this study. Under batch culture conditions, Bacillus thuringiensis EGU45 and Bacillus cereus EGU44 evolved 1.67–1.92 mol H2/mol glucose, respectively during the initial 3 days of incubation at 37°C. In the next 2 days, the residual glucose solutions along with B. thuringiensis EGU45 shaken at 200 rpm was found to produce PHB yield of 11.3% of dry cell mass. This is the first report among the non-photosynthetic microbes, where the Bacillus spp.—B. thuringiensis and B. cereus strains have been shown to produce H2 and PHB in same medium under different conditions.

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Acknowledgments

The authors wish to thank Director of Institute of Genomics and Integrative Biology (IGIB), CSIR and Department of Biotechnology, Government of India for providing the necessary funds, facilities and moral support. S.K.S. Patel and M. Singh are thankful to CSIR and UGC for Senior Research Fellowships.

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Authors and Affiliations

  1. Microbial Biotechnology and Genomics, Institute of Genomics and Integrative Biology (IGIB), CSIR, Delhi University Campus, Mall Road, Delhi, 110007, India

    Sanjay K. S. Patel, Mamtesh Singh & Vipin C. Kalia

  2. Department of Biotechnology, University of Pune, Pune, 411007, India

    Sanjay K. S. Patel & Mamtesh Singh

Authors
  1. Sanjay K. S. Patel
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  2. Mamtesh Singh
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  3. Vipin C. Kalia
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Correspondence to Vipin C. Kalia.

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Sanjay K. S. Patel and Mamtesh Singh contributed equally to this paper.

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Patel, S.K.S., Singh, M. & Kalia, V.C. Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System. Indian J Microbiol 51, 418–423 (2011). https://doi.org/10.1007/s12088-011-0236-9

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  • DOI: https://doi.org/10.1007/s12088-011-0236-9

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