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Identification of Thermophilic Bacterial Strains Producing Thermotolerant Hydrolytic Enzymes from Manure Compost

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Abstract

Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60–65°C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, α-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications.

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Acknowledgments

This work was supported through grants from NSERC. D.M.C. acknowledges the support of a PROTEO scholarship. Special thanks to Sandra Isabel for providing guidance in phylogenetic analysis, and to Dr Martin Kalmokoff (Agriculture Canada) for editorial review of the manuscript.

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Authors and Affiliations

  1. Département de Chimie-Biologie, Université du Québec à Trois-Rivières, 3351 Boul. Des Forges, C.P. 500, Trois-Rivières (Québec), G9A 5H7, Canada

    David M. Charbonneau, Fatma Meddeb-Mouelhi, Marc Sirois & Marc Beauregard

  2. Buckman Laboratories of Canada, 351, Joseph-Carrier, Vaudreuil-Dorion (Québec), J7V 5V5, Canada

    Fatma Meddeb-Mouelhi

  3. Centre de Recherche en Infectiologie de l’Université Laval, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, 2705 Boul. Laurier, RC-709, Ste-Foy (Québec), G1V 4G2, Canada

    Maurice Boissinot

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  1. David M. Charbonneau
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  2. Fatma Meddeb-Mouelhi
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  3. Maurice Boissinot
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  4. Marc Sirois
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  5. Marc Beauregard
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Correspondence to Marc Beauregard.

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Charbonneau, D.M., Meddeb-Mouelhi, F., Boissinot, M. et al. Identification of Thermophilic Bacterial Strains Producing Thermotolerant Hydrolytic Enzymes from Manure Compost. Indian J Microbiol 52, 41–47 (2012). https://doi.org/10.1007/s12088-011-0156-8

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  • DOI: https://doi.org/10.1007/s12088-011-0156-8

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