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Isolation, characterization and identification of cellulose-degrading bacteria for composting of agro-wastes

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Abstract

An endeavour is underway to isolate native cellulose-degrading bacteria from different sources in order to have them used in composting of organic waste. On the basis of cellulase production and growth in different environmental circumstances, five isolates were selected. The 16S rRNA genetic sequence of five isolates was submitted to the National Center for Biotechnology Information (NCBI), which assigned accession numbers ON150745, ON178665, ON725042, ON479186 and ON142173 for CBD4, CBG3, CBG2, CBC9 and CBG4 in accordance. The growth of CBD4, CBG2, CBG3 and CBG4 reached the stationary phase at 24 h and lasted up to 78 h, but in case of CBC9 stationary phase of growth lies between 32 and 50 h of incubation which was less than other bacterial strains. The strains’ optimum CMCase production conditions were 5% inoculum, a pH of 7.5, the temperature of 35° C for CBD4 and CBC9; 4% inoculum, a pH of 6.5 and temperature of 35° C for CBG2, CBG3; and 3% inoculum with same pH and temperature for CBG4. The CMCase production at respective optimum conditions was highest in the case of CBG2 strain. The CMCase reaction for all the strains was highest at 50 °C and decreased in higher or lower temperatures. The reaction of cellulase produced by strain CBG2 at optimum pH and temperature was highest. In this study, the cellulolytic potential of the CBC9 strain (B. xiamenensis) was examined for the first time.

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Acknowledgements

The infrastructure support of Odisha University of Agriculture and Technology is duly acknowledged.

Funding

The financial support of Odisha University of Agriculture and Technology is duly acknowledged.

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Authors and Affiliations

  1. Department of Soil Science and Agricultural Chemistry, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, India

    Kshitipati Padhan, Ranjan Kumar Patra, Debadatta Sethi, Narayan Panda, Sanjib Kumar Sahoo & Sushanta Kumar Pattanayak

  2. Department of Plant Pathology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, India

    Akshaya Kumar Senapati

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Contributions

Kshitipati Padhan, Ranjan Kumar Patra: conceptualization, writing—original draft, review and editing; Akshaya Kumar Senapati: conceptualization, writing—review and editing; Debadatta Sethi: writing—original draft, writing—review and editing; Narayan Panda: writing—review and editing; Sanjib Kumar Sahoo: writing—review and editing; Sushanta Kumar Pattanayak: conceptualization, writing—review and editing.

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Correspondence to Debadatta Sethi.

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Padhan, K., Patra, R.K., Sethi, D. et al. Isolation, characterization and identification of cellulose-degrading bacteria for composting of agro-wastes. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04087-y

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