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Biosurfactant Production by Pseudomonas aeruginosa from Renewable Resources

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Abstract

This study deals with production and characterization of biosurfactant from renewable resources by Pseudomonas aeruginosa. Biosurfactant production was carried out in 3L fermentor using waste motor lubricant oil and peanut oil cake. Maximum biomass (11.6 mg/ml) and biosurfactant production (8.6 mg/ml) occurred with peanut oil cake at 120 and 132 h respectively. Characterization of the biosurfactant revealed that, it is a lipopeptide with chemical composition of protein (50.2%) and lipid (49.8%). The biosurfactant (1 mg/ml) was able to emulsify waste motor lubricant oil, crude oil, peanut oil, kerosene, diesel, xylene, naphthalene and anthracene, comparatively the emulsification activity was higher than the activity found with Triton X-100 (1 mg/ml). Results obtained in the present study showed the possibility of biosurfactant production using renewable, relatively inexpensive and easily available resources. Emulsification activity found with the biosurfactant against different hydrocarbons showed its possible application in bioremediation of environments polluted with various hydrocarbons.

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Acknowledgment

The authors are thankful to the authorities of Annamalai University for providing the facilities and Department of Ocean Development and Council of Scientific and Industrial Research, Government of India for their financial support to carry out the work.

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Authors and Affiliations

  1. Department of Chemical and Biological Sciences, Polytechnic Institute of New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA

    R. Thavasi

  2. CAS in Marine Biology, Annamalai University, Parangipettai, Chidambaram, Tamil Nadu, 608 502, India

    V. R. M. Subramanyam Nambaru, S. Jayalakshmi & T. Balasubramanian

  3. School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, Northern Ireland, UK

    Ibrahim M. Banat

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  1. R. Thavasi
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  2. V. R. M. Subramanyam Nambaru
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  3. S. Jayalakshmi
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  4. T. Balasubramanian
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  5. Ibrahim M. Banat
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Correspondence to R. Thavasi.

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Thavasi, R., Subramanyam Nambaru, V.R.M., Jayalakshmi, S. et al. Biosurfactant Production by Pseudomonas aeruginosa from Renewable Resources. Indian J Microbiol 51, 30–36 (2011). https://doi.org/10.1007/s12088-011-0076-7

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  • DOI: https://doi.org/10.1007/s12088-011-0076-7

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