Summary
Enzymes catalyzing ATP-PPi exchange reactions mediated by specific amino acids were found inB. subtilis lysates partially purified by gel permeation. The nature of these amino acids varied according to the stage of cell growth: activation of surfactin amino acids was observed during surfactin synthesis; activation of iturin amino acids was not detected at the begining of surfactin synthesis but appeared during iturin synthesis.
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Besson, F., Michel, G. Biosynthesis of iturin and surfactin byBacillus subtilis: Evidence for amino acid activating enzymes. Biotechnol Lett 14, 1013–1018 (1992). https://doi.org/10.1007/BF01021050
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DOI: https://doi.org/10.1007/BF01021050