Skip to main content

Advertisement

SpringerLink
Log in

Cyanobacterial diversity in the rhizosphere of rice and its ecological significance

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

This investigation was undertaken to characterize the abundance and genera-wise diversity of cyanobacteria in the rice rhizosphere and nitrogen-fixing ability of the isolated strains. The cyanobacterial strains belonging to the genera Nostoc and Anabaena comprised 80% of the rhizosphere isolates, which were also efficient in enhancing the germination and growth of wheat seeds and exhibited significantly high protein accumulation and IAA production. Distinct profiles for the cyanobacterial strains were obtained on amplification with extended Hip 1 primer — HipTG, indicative of the diversity among these strains. Our investigation helped in identifying promising cyanobacterial isolates from the rhizosphere of rice, which can be utilized in developing efficient plant growth promoting cyanobacterial inoculants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kulasooriya SA (1998) Cyanobacteria and Azolla as biofertilizer for rice. In: Cyanobacterial Biotechnology (Subramanian GS, Kaushik BD, Venkataraman GS eds), Oxford and IBH Publishing Co. Pvt. Ltd.: New Delhi, pp 201–209

    Google Scholar 

  2. Vaishampayan A, Sinha RP, Hader DP, Dey T, Gupta AK, Bhan U and Rao AL (2001) Cyanobacterial biofertilizers in rice agriculture. Bot Rev 67:453–516

    Article  Google Scholar 

  3. Whitton BA (2000) Soils and rice fields. In: The Ecology of Cyanobacteria (Whitton BA and Potts M eds), Kluwer Academic Publishers, Dordrecht, Netherlands, pp 233–255

    Google Scholar 

  4. Frieberg E (1998) Microclimatic parameters influencing nitrogen fixation in the phyllosphere in a Costa Rican premontane rain forest. Oecologia 117:9–18

    Article  Google Scholar 

  5. Gantar M, Kerby NW, Rowell P and Obreht Z, (1991) Colonisation of wheat (Triticum vulgare L.) by nitrogen fixing cyanobacteria: I. A survey of soil cyanobacterial isolates forming associations with roots. New Phytol 118: 477–483

    Article  Google Scholar 

  6. Whitton BA, Aziz A, Kawecka B and Rother JA (1988) Ecology of deep water rice fields in Bangladesh. 3. Associated algae and saprophytes. Hydrobiologia 169:31–42

    Article  Google Scholar 

  7. Watanabe I, Lee KK, Alimango BV, Sato M, Del Rosario DC, De Guzman MR (1977) Biological nitrogen fixation in paddy field studied by in situ acetylene-reduction assays. IRRI Res Paper Ser, 3:16

    Google Scholar 

  8. Wada H, Panichsakapatana S, Kimura M and Takai Y (1978) Nitrogen fixation in paddy soils. Part I. Controlling factors affecting N2 fixation. Soil Sci Plant Nutr 24:13–17

    Google Scholar 

  9. Malik KA, Mirza MS, Hassan U, Mehnaz S, Haurat J, Bally R and Normand P (2002) The role of plant associated beneficial bacteria in rice wheat cropping system. In: Biofertilizers in action (Kennedy IR and Choudhary ATMA eds), Rural Industries Research and Development Corporation, Canberra, pp 45–54

    Google Scholar 

  10. Rai AN and Bergman B (2002) Creation of new nitrogen fixing cyanobacteria associations. Biol. Environ. Proc Royal Irish Acad 102B:65–68

    Article  Google Scholar 

  11. Nilsson M, Rasmussen U and Bergman B (2005) Competition among symbiotic cyanobacterial Nostoc strains forming artificial associations with rice (Oryza sativa). FEMS Microbiol Lett 245:139–144

    Article  PubMed  CAS  Google Scholar 

  12. Nilsson M, Bhatacharaya J, Rai AN and Bergman B (2002) Colonization of root of rice (Oryza sativa) by symbiotic Nostoc strains. New Phytol 156:517–525

    Article  Google Scholar 

  13. Prasanna R, Pabby, A, Saxena S and Singh PK (2004) Modulation of pigment profiles of Calothrix elenkenii in response to environmental stresses. J Plant Physiol 161:1125–1132

    Article  PubMed  CAS  Google Scholar 

  14. Sergeeva E, Liaimer A and Bergman B (2002) Evidence for the production of the phytohormone indole-3-acetic acid by cyanobacteria. Planta 215:229–238

    Article  PubMed  CAS  Google Scholar 

  15. Rippka R, Deruelles J, Waterbury JB, Herdman M and Stanier RY (1979) genetic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111: 1–61

    Google Scholar 

  16. Stanier RY, Kunisawa R, Mandel M and Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35: 171–205

    PubMed  CAS  Google Scholar 

  17. Desikachary TV (1959) Cyanophyta. Indian Council of Agricultural Research, New Delhi

    Google Scholar 

  18. Black CA (1965) Methods of Soil Analysis (Part 1). American Soc. Agron., USA

    Google Scholar 

  19. Herbert D, Phipps P J and Strange RE (1971) Chemical analysis of microbial cells. In: Methods in Microbiology, Vol. V.B, (Norris JR, Ribbons DW eds.) Academic Press, New York, pp 209–344

    Chapter  Google Scholar 

  20. Gordon AS and Weber RP (1951) Colorimetric estimation of indole acetic acid. Plant Physiol 26:192–195

    Article  PubMed  CAS  Google Scholar 

  21. Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322

    CAS  Google Scholar 

  22. Prasanna, R, Tripathi, U, Dominic TK, Singh AK, Yadav, AK and Singh PK (2003) An improvised technique for measurement of nitrogen fixation by blue green algae and Azolla using intact soil cores. Expl Agric 39:145–150

    Article  CAS  Google Scholar 

  23. Rasmussen U and Svenning MM (1998) Fingerprinting of cyanobacteria based on PCR with primers derived from short and long tandemly repeated repetitive sequences. Appl Environ Microbiol 64:265–272

    PubMed  CAS  Google Scholar 

  24. Zheng W, Song T, Bao X. and Bergman B (2002) High cyanobacterial diversity in coralloid roots of cycads revealed by PCR fingerprinting. FEMS Microbiol Lett 40:215–222

    CAS  Google Scholar 

  25. Venkataraman GS (1972) Algal biofertilizers and Rice cultivation. Today and Tomorrow Publishers, Delhi

    Google Scholar 

  26. Nayak S, Prasanna R, Dominic TK and Singh PK (2001) Floristic abundance and relative distribution of different cyanobacterial genera in rice field soil at different crop growth stages. Phykos 40:14–21

    Google Scholar 

  27. Nayak S, Prasanna R, Pabby A, Dominic TK and Singh PK (2004) Effect of BGA- Azolla biofertilizers on nitrogen fixation and chlorophyll accumulation at different depths in soil cores. Biol Fertil Soils 40:67–72

    Article  CAS  Google Scholar 

  28. Song T, Martensson L, Eriksson T, Zheng W and Rasmussen U (2005) Biodiversity and seasonal variation of the cyanobacterial assemblage in a rice paddy field in Fujian, China. FEMS Microbiol Ecol 54:131–140

    Article  PubMed  CAS  Google Scholar 

  29. Prasanna R, Saxena AK, Jaiswal P and Nayak S (2006) Development of alternative support system for viable count of cyanobacteria by MPN method. Folia Microbiol 51:455–458

    Article  CAS  Google Scholar 

  30. Mishra S and Kaushik BD (1989) Growth promoting substances of cyanobacteria. I. Vitamins and their influence on rice plant. Proc Indian Sci Acad B55:295–300

    Google Scholar 

  31. Pedurand P and Reynaud PA (1987) Do cyanobacteria enhance germination and growth of rice? Plant Soil 101: 235–240

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre for Conservation and Utilization of Blue-Green Algae, Indian Agricultural Research Institute (IARI), New Delhi, 110 012, India

    Radha Prasanna, Pranita Jaiswal, Saswati Nayak & Anjuli Sood

  2. Division of Microbiology, IARI, New Delhi, 110 012, India

    Brahma D. Kaushik

Authors
  1. Radha Prasanna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pranita Jaiswal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saswati Nayak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anjuli Sood
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Brahma D. Kaushik
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Radha Prasanna.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prasanna, R., Jaiswal, P., Nayak, S. et al. Cyanobacterial diversity in the rhizosphere of rice and its ecological significance. Indian J Microbiol 49, 89–97 (2009). https://doi.org/10.1007/s12088-009-0009-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-009-0009-x

Keywords

Search

Navigation