Skip to main content

Advertisement

SpringerLink
Log in

Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Diversified group of bacteria were screened for carbonic anhydrase (CA) activity. Significant CA activity was found in crude enzyme extracts of Enterobacter and Aeromonas isolates while minimal or negligible CA activity was observed in case of Shigella and Klebsiella spp. Optimization and characterization study of potent CA producing isolates revealed that the maximum enzyme activity of 3.86 EU/ml was observed in E. taylorae and the optimum pH range for enzyme stability was found to be 7.5–9.0 along with an optimum temperature range of 35–50 °C. The molecular mass of CA was 29-kDa indicating α-type with periplasmic and cytosolic location. Present investigation for the first time reports CA in diversified genus and optimized parameters for enhanced production of CA in Enterobacter sp. & Aeromonas sp. from fresh water bodies that inturn lay down grounds for exploitation of CA from E. taylorae as an efficient catalyst for CO2 sequestration within a bioreactor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kupriyanova E, Villarejo A, Markelova A, Gerasimenko L, Zavarzin G, Samuelsson G, Los DA & Pronina N (2007) Extracellular Carbonic anhydrases of stromatolite forming cyanobacterium Microcoleus cathonoplastes. Microbiology 153:1149–156

    Article  PubMed  CAS  Google Scholar 

  2. Bond GM, Stranger J, Branchvold DK, Sinsek FA, Medina NG & Egeland G (1999) Development of integrated system for biomimetic CO2 Sequestration using the enzyme carbonic anhydrase. Energy & Fuels 15:309–31

    Article  CAS  Google Scholar 

  3. Smith KS & Ferry JG (2000) Prokaryotic Carbonic anhydrases. FEMS Microbiol Rev 24:335–366

    Article  PubMed  CAS  Google Scholar 

  4. Adler L, Brundell J, Falkbring SO & Nyman PO (1972) Carbonic anhydrase from Nisseria sicca strain 6021. Bacterial Growth and Purification of enzyme. Biochemica et Biophysica Acta 284:313–323

    Google Scholar 

  5. Alber BE & Ferry JG (1994) A carbonic anhydrase from the archaeon Methanosarcina thermophila. Proc Natl Acad Sci USA 91:6909–6913

    Article  PubMed  CAS  Google Scholar 

  6. Braus-Stromeyer S, Schnappauf G, Braus GH, Gobner A & Drake HL (1997) Carbonic anhydrase in Acetobacterium woodii. J Bacteriol 179:7197–7200

    PubMed  CAS  Google Scholar 

  7. Gill SR, Fedorka PJ, Tweten RK & Sleeper BP (1984) Purification and Properties of Carbonic anhydrase of Rhodospirullum rubrum. Arch Microbiol 138:113–118

    Article  CAS  Google Scholar 

  8. Yagawa Y, Shiraiwa Y & Miyachi S (1984) Carbonic anhydrase from the blue green algae (cyanobacterium) Anabaena variabilis. Plant Cell Physiol 25:775–783

    CAS  Google Scholar 

  9. Buzolyova LS & Somov G (1999) Autotrophic assimilation of CO2 and C1 compounds by pathogenic bacteria. Biochem (Moscow) 64 (10):1357–1361

    Google Scholar 

  10. Smith KS, Zakubzick C, Whittam TS & Ferry JG (1999) Carbonic anhydrase is an ancient enzyme wide spread in prokaryotes. Proc Natl Acad Sci USA 96:15184–15189

    Article  PubMed  CAS  Google Scholar 

  11. Dean CR & Ward OP (1992) The use of EDTA or polymixin with lysozyme for the recovery of intracellular products from Eschericia coli. Biotechnology Techniques 6 (2):133–138

    Article  CAS  Google Scholar 

  12. Wilbur KM & Anderson NG (1948) Electrometric and Colorimetric determination of Carbonic anhydrase. J Biolchem 176:147–154

    CAS  Google Scholar 

  13. Lowry OH, Rosenbrough NJ, Forr AL & Randall RJ (1951) Protein measurement with Folin Phenol Reagent. J Biolchem 193:265–275

    CAS  Google Scholar 

  14. Smith KS & Ferry JG (1999) A plant type (β-Class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum. J Bacteriol 181:6247–6253

    PubMed  CAS  Google Scholar 

  15. Ames G, Prody FL & Kustu S (1984) Simple, rapid and quantitative release of periplasmic proteins by choloroform. J Bacteriol 160:1181–1183

    PubMed  CAS  Google Scholar 

  16. Russel M & Model P (1982) Filamentous phage precoat is an integral membrane protein: analysis by a new method of membrane preparation. Cell 28:177–184

    Article  PubMed  CAS  Google Scholar 

  17. Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature (London) 227:680–685

    Article  CAS  Google Scholar 

  18. Merlin C, Masters M, Mcteer S & Corlson A (2003) Why is carbonic anhydrase essential to E. coli. J Bacteriol 185(21): 6415–6424

    Article  PubMed  CAS  Google Scholar 

  19. Charles HP & Roberts (1968) Carbon dioxide as a growth factor for mutants of E. coli. J Gen Microbiol 51:211–224

    PubMed  CAS  Google Scholar 

  20. Demir N, Demir Y & Coskum P (2001) Purification and characterization of carbonic anhydrase from human erythrocyte plasma membrane. Turk J Med Sci 31:477–482

    CAS  Google Scholar 

  21. Demir Y, Demir N, Nadarglu H & Bakan E (2000) Purification and characterization of carbonic anhydrase from bovine erythrocyte plasma membrane. Biochem & Biotechnol 30 (1):49–59

    Article  CAS  Google Scholar 

  22. Stahler MF, Ganter L, Katherin L, Manfred K & Stephen B (2005) Mutational analysis of Helicobacter pylori carbonic anhydrases. FEMS Immunol Medical Microbiol 44:183–189

    Article  CAS  Google Scholar 

  23. Park IS & Hausinger RP (1995) Requirement of carbon dioxide for in vitro assembly of urease nickel metallocenter. Sci 267:1156–1158

    Article  CAS  Google Scholar 

  24. Earnhardt JN, Qian MC, Lakkis MM, Bergenhem NC, Laipis PY, Tashian RE & Silverman DM (1998) The catalytic properties of marine carbonic anhydrase VII. Biochem 37:10837–10845

    Article  CAS  Google Scholar 

  25. Chirica LC, Elleby B, Jonsson BH & Lindskog S (1997) The complete sequence, expression in E. coli, purification and some properties of carbonic anhydrase from Nisseria gonorrhoeae. Eur J Biochem 244:755–760

    Article  PubMed  CAS  Google Scholar 

  26. Chirica L, Peteresson C, Hurtig M, Jonsson BH, Boren T & Lindskog S (2002) Expression and localization of α and β Carbonic anhydrase in Helicobacter pylori. Biochemica and Biophysica Acta 160:192–199

    Google Scholar 

  27. Li W, Yu LJ, Wu Y, Jia LP & Yuan DX (2007) Enhancement of calcium release from limestone by microbial extracellular carbonic anhydrase. Bioresource Tech 98:950–953

    Article  CAS  Google Scholar 

  28. Pushkas LG, Inui M, Zahan K & Yukawa H (2000) A periplasmic α-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology 146:2957–2966

    Google Scholar 

  29. Rawat M & Moroney JV (1995) The regulation of carbonic anhydrase and ribulose 1,5 biphosphate carboxylase / Oxygenase activase by light and CO2 in Chlamydomonas reinhardtii. Plant Physiol 109:937–944

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bacteriology Laboratory, Department of P.G studies and Research in Biological Sciences, R.D University, Jabalpur, 482 001 (M.P.), India

    Anjana Sharma, Abhishek Bhattacharya, Rajesh Pujari & Ankita Shrivastava

Authors
  1. Anjana Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Abhishek Bhattacharya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rajesh Pujari
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ankita Shrivastava
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anjana Sharma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharma, A., Bhattacharya, A., Pujari, R. et al. Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration. Indian J Microbiol 48, 365–371 (2008). https://doi.org/10.1007/s12088-008-0045-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-008-0045-y

Keywords

Search

Navigation