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The use of EDTA or polymyxin with lysozyme for the recovery of intracellular products fromEscherichia. coli

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Biotechnology Techniques

Summary

Escherichia coli cells taken from exponential and late stationary (or decline) phases of culture were very susceptible to lysis by EDTA/lysozyme. Log phase cells were most susceptible to lysis by polymyxin/lysozyme. Treatment ofE. coli with EDTA and lysozyme compared favourably with sonication as a method for release of intracellular protein. Concentration ranges for optimal lysis were 100–800 μg/ml for EDTA and 25–50 μg/ml for lysozyme.

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Authors and Affiliations

  1. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    C. R. Dean & O. P. Ward

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  1. C. R. Dean
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  2. O. P. Ward
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Dean, C.R., Ward, O.P. The use of EDTA or polymyxin with lysozyme for the recovery of intracellular products fromEscherichia. coli . Biotechnol Tech 6, 133–138 (1992). https://doi.org/10.1007/BF02438819

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  • DOI: https://doi.org/10.1007/BF02438819

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