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Effects of homocysteine on adipocyte differentiation and CD36 gene expression in 3T3-L1 adipocytes

  • RESEARCH ARTICLE
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Journal of Cell Communication and Signaling Aims and scope

Abstract

The aim of this study was to investigate the effects of homocysteine (Hcy), a risk factor for cardiovascular diseases, hypertension, stroke and obesity, on expression of CD36 that regulates uptake of oxidized low-density lipoprotein (Ox-LDL) by adipocytes and differentiation of 3T3-L1 cells to adipocytes. Cell viability was determined using MTT assay, and density of triglycerides were measured with Oil Red O staining. The expression levels of CD36 were analyzed using SYBR green assay by quantitative RT-PCR. Our results showed that the addition of Hcy inhibited differentiation of 3T3-L1 preadipocytes in a dose-dependent manner without a significant cell toxicity (p < 0.05). Percentage CD36 gene expression increased in the Hcy treatment groups, but not statistically significantly (p > 0.05) compared to differentiated adipocytes. Hcy reduced adipocyte differentiation, but had no effect on the expression level of CD36 in vitro conditions. The effect of Hcy on uptake and clearance of Ox-LDL by adipose tissue now needs to be investigated in vivo.

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Abbreviations

Hcy:

Homocysteine

Ox-LDL:

Oxidized low-density lipoprotein

IBMX:

Isobutylmethyl xanthine

PPARγ:

Peroxisome proliferator-activated receptor gamma

C/EBPα:

CCAAT/enhancer binding protein alpha

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Acknowledgments

This study was supported by the Karadeniz Technical University Research Fund.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

    Ahmet Mentese & Ahmet Alver

  2. Department of Nursing, School of Health Services, Recep Tayyip Erdogan University, Rize, Turkey

    Aysegul Sumer

  3. Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, Trabzon, Turkey

    Selim Demir

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  1. Ahmet Mentese
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  2. Ahmet Alver
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  3. Aysegul Sumer
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  4. Selim Demir
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Correspondence to Ahmet Mentese.

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None of the authors had any financial or personal relationships with other individuals or organizations that might inappropriately influence their work during the submission process.

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Mentese, A., Alver, A., Sumer, A. et al. Effects of homocysteine on adipocyte differentiation and CD36 gene expression in 3T3-L1 adipocytes. J. Cell Commun. Signal. 10, 55–60 (2016). https://doi.org/10.1007/s12079-015-0316-4

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  • DOI: https://doi.org/10.1007/s12079-015-0316-4

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