Abstract
Obesity is characterized by a chronic inflammatory process, with an increased volume of total adipose tissue, especially visceral, which secretes pro-inflammatory cytokines such as TNF-α and IL-6. Hepcidin (Hpc), a main iron metabolism regulator, is synthetized by an IL-6 stimuli, among others, in liver and adipose tissue, favoring an association between the inflammatory process and iron metabolism. Still there are questions remain regarding the interaction of these factors. Our aim was to study the effect of a macrophage-conditioned medium (MCM) on adipocyte cells challenged with glucose and/or iron. We studied the mRNA relative abundance of genes related to inflammation in differentiated 3T3-L1 cells challenged with Fe (40 µM), glucose (20 mM) or Fe/glucose (40 µM/20 mM) with or without MCM for 24 h. We also measured the intracellular iron levels under these conditions. Our results showed that when adipocytes were challenged with MCM, glucose and/or Fe, the intracellular iron and mRNA levels of pro-inflammatory cytokines increased. These responses were higher when all the stimuli were combined with MCM from macrophages. Thus, we showed that combined high glucose/high Fe alone or with MCM may contribute to an increase on intracellular iron and inflammatory response in 3T3-L1 differentiated cells, by increased mRNA levels of IL-6, TNF-α, MCP-1, Hpc and reducing adiponectin levels, enhancing the inflammatory processes.
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Acknowledgements
This research was supported by the FONDECYT No 1110080 to M Arredondo and DIUBB No 1674204/I to L Briones.
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LB conception and design of research, performed experiments, analysis of data and drafted manuscript. MAG, conception and design of research, interpreted results of experiments and drafted manuscript. FP interpreted results of experiments, analysis of data and drafted manuscript MAO conception and design of research, interpreted results of experiments, prepared figures, analysis of data and drafted manuscript. LB, MAG, FP and MAO edited and revised manuscript and approved final version of manuscript.
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Briones, L., Andrews, M., Pizarro, F. et al. Expression of genes associated with inflammation and iron metabolism in 3T3-L1 cells induced with macrophages-conditioned medium, glucose and iron. Biometals 31, 595–604 (2018). https://doi.org/10.1007/s10534-018-0108-4
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DOI: https://doi.org/10.1007/s10534-018-0108-4