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Discerning molecular diversity and association mapping for phenological, physiological and yield traits under high temperature stress in chickpea (Cicer arietinum L.)

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Abstract

High temperature (HT) stress is assuming serious production constraint for chickpea production worldwide. A collection of 182 diverse chickpea genotypes was assessed for genetic variation in 15 traits including phenological, physiological and yield-related traits under both normal sown (NS) and late sown (LS) conditions for two years 2017–2018 and 2018–2019, which revealed significant variation for all the traits. Association mapping of chickpea genotypes was also conducted with 120 simple sequence repeat markers distributed across all the chickpea chromosomes to discern the molecular diversity and to capture the significant marker-trait association (MTA). MTA analysis based on mixed linear model (MLM) revealed a total of 24 and 14 significant associations for various traits evaluated under NS conditions in 2017 and 2018, respectively. Similarly, a total of 17 and 34 significant associations for various traits were also recorded under LS conditions in 2018 and 2019, respectively. Notably, ICCM0297, NCPGR150, TAA160 and NCPGR156 markers showed significant MTA under both NS and LS conditions and GA11 exhibited significant MTA for filled pod% under late sown condition for both years. Thus, these markers could be useful for genomics-assisted breeding for developing heat-tolerant chickpea genotype.

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Acknowledgements

The authors acknowledge support from Indian Council of Agricultural Research (ICAR), India. This research is funded by National Initiative Crop Resilience (NICRA) project, ICAR, New Delhi.

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Authors and Affiliations

  1. Indian Institute of Pulses Research (IIPR), Kanpur, 208 024, India

    Uday Chand Jha, Anurag Kumar, Sanjeev Gupta, Parthasarathi Basu & Narendra Pratap Singh

  2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Rintu Jha

  3. National Institute of Plant Genome Research (NIPGR), New Delhi, 110 067, India

    Virevol Thakro & Swarup K. Parida

  4. Department of Botany, Panjab University, Chandigarh, 160 014, India

    Harsh Nayyar

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  1. Uday Chand Jha
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Corresponding editor: Manoj Prasad

UCJ conceived the idea and wrote the MS. RJ conducted the SSR analysis, AK collected the phenotypic data, PSB, HN performed the statistical analysis, VT, SKP performed the candidate gene analysis. SG and NP edited the MS. All authors read and approved the final manuscript.

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Jha, U.C., Jha, R., Thakro, V. et al. Discerning molecular diversity and association mapping for phenological, physiological and yield traits under high temperature stress in chickpea (Cicer arietinum L.). J Genet 100, 4 (2021). https://doi.org/10.1007/s12041-020-01254-2

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