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Identification and expression analysis of primary auxin-responsive Aux/IAA gene family in cucumber (Cucumis sativus)

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Abstract

Aux/IAA is an important gene family involved in many aspects of growth and development. Aux/IAA proteins are short-lived nuclear proteins that are induced primarily by various phytohormones. In this study, 29 Aux/IAA family genes (CsIAA01–CsIAA29) were identified and characterized in cucumber, including gene structures, phylogenetic relationships, conserved protein motifs and chromosomal locations. These genes show distinct organizational patterns of their putative motifs. The distributions of the genes vary: except for five CsIAA genes in cucumber that were not located, seven CsIAA genes were found on scaffold, while the other 17 CsIAA genes were distributed on seven other chromosomes. Based on a phylogenetic analysis of the Aux/IAA protein sequences from cucumber, Arabidopsis and other plants, the Aux/IAA genes in cucumber were categorized into seven subfamilies. To investigate whether the expression of CsIAA genes is associated with auxin induction, their transcript levels were monitored in seedlings treated with IAA (indole-3-acetic acid), and their expression patterns were analysed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that 11/29 CsIAA genes were expressed in leaves whether treated with IAA or not and the time course of processing and compared with the control, five CsIAA genes showed low expression only after 60 min treatment with IAA, while 11 genes showed no expression. These results provide useful information for further functional analysis of Aux/IAA gene family in cucumber.

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Acknowledgements

This research is supported by grants from the Higher Education Revitalization Project of Anhui Province (2013zdjy057), the Scientific Research Foundation for the Stability and Introduction of the Talents (wd2011-14), the Natural Science Foundation of Anhui Province (1208085MC36) and the Natural Science Research Foundation of Anhui Province (KJ2011A110). We thank members of the Key Laboratory of Crop Biology of Anhui Province for their assistance in this study.

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Authors and Affiliations

  1. Anhui Province Key Laboratory of Crop Biology, School of Life Science, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    DEFANG GAN & YANG ZHAO

  2. School of Horticulture, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    DEFANG GAN, DAN ZHUANG, FEI DING & ZHENZHOU YU

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  1. DEFANG GAN
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  2. DAN ZHUANG
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  3. FEI DING
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  4. ZHENZHOU YU
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  5. YANG ZHAO
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Correspondence to DEFANG GAN.

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[Gan D., Zhuang D., Ding F., Yu Z. and Zhao Y. 2013 Identification and expression analysis of primary auxin-responsive Aux/IAA gene family in cucumber (Cucumis sativus). J. Genet. 92, xx–xx]

Defang Gan and Dan Zhuang conributed equally to this work

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GAN, D., ZHUANG, D., DING, F. et al. Identification and expression analysis of primary auxin-responsive Aux/IAA gene family in cucumber (Cucumis sativus) . J Genet 92, 513–521 (2013). https://doi.org/10.1007/s12041-013-0306-3

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