Skip to main content
SpringerLink
Log in

Mapping of shoot fly tolerance loci in sorghum using SSR markers

  • Research Article
  • Published:
Journal of Genetics Aims and scope Submit manuscript

Abstract

Sorghum (Sorghum bicolor (L.) Moench) is one of the most important crops in the semiarid regions of the world. One of the important biotic constraints to sorghum production in India is the shoot fly which attacks sorghum at the seedling stage. Identification of the genomic regions containing quantitative trait loci (QTLs) for resistance to shoot fly and the linked markers can facilitate sorghum improvement programmes through marker-assisted selection. A simple sequence repeat (SSR) marker- based skeleton linkage map of two linkage groups of sorghum was constructed in a population of 135 recombinant inbred lines (RIL) derived from a cross between IS18551 (resistant to shoot fly) and 296B (susceptible to shoot fly). A total of 14 SSR markers, seven each on linkage groups A and C were mapped. Using data of different shoot fly resistance component traits, one QTL which is common for glossiness, oviposition and dead hearts was detected following composite interval mapping (CIM) on linkage group A. The phenotypic variation explained by this QTL ranged from 3.8%–6.3%. Besides the QTL detected by CIM, two more QTLs were detected following multi-trait composite interval mapping (MCIM), one each on linkage groups A and C for the combinations of traits which were correlated with each other. Results of the present study are novel as we could find out the QTLs governing more than one trait (pleiotropic QTLs). The identification of pleiotropic QTLs will help in improvement of more than one trait at a time with the help of the same linked markers. For all the QTLs, the resistant parent IS18551 contributed resistant alleles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aruna C. and Padmaja P. G. 2009 Evaluation of genetic potential of shoot fly resistant sources in sorghum Sorghum bicolor (L.) Moench. J. Agric. Sci. 147, 71–80.

    Article  CAS  Google Scholar 

  • Bantilan M. C. S., Deb U. K., Gowda C. L. L., Reddy B. V. S., Obilana A. B. and Evenson R. E. (ed.) 2004 Sorghum genetic enhancement: research process, dissemination and impacts, pp. 320. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India.

  • Bhattramakki D., Dong J., Chhabra A. K. and Hart G. E. 2000 An integrated SSR and RFLP linkage map of Sorghum bicolor (L.) Moench. Genome 43, 988–1002.

    PubMed  CAS  Google Scholar 

  • Chen Y. and Lubberstedt T. 2010 Molecular basis of trait correlations. Trends Plant Sci. 15, 454–461.

    Article  PubMed  CAS  Google Scholar 

  • Deeming J. C. 1971 Some species of Atherigona rondani (Diptera: Muscidae) from northern Nigeria, with special reference to those injurious to cereal crops. Bull. Entomol. Res. 61, 133–190.

    Article  Google Scholar 

  • Folkertsma R. T., Sajjanar G. M., Reddy B. V. S., Sharma H. C. and Hash C. T. 2003 Genetic mapping of QTL associated with sorghum shoot fly (Atherigona soccata) resistance in sorghum (Sorghum bicolor). In Final abstracts guide, plant and animal genome XI, p. 462. Town and Country Hotel, San Diego, USA (http://www.intlpag.org/11/abstracts/P5dP462XI.html).

  • Gupta P. K., Kumar J., Mir R. R. and Kumar A. 2010 Marker-assisted selection as a component of conventional plant breeding. Plant Breed. Rev. 33, 145–217.

    Article  Google Scholar 

  • Hallali M. S., Gowda B. T. S., Kulkarni K. A. and Goud J. V. 1982 Inheritance of resistance to shootfly (Atherigona soccata Rond.) in sorghum. SABRAO J. 14, 165–170.

    Google Scholar 

  • Haussmann B. I. G., Hess D. E., Seetharama N., Welz H. G. and Geiger H. H. 2002 Construction of a combined sorghum linkage map from two recombinant inbred populations using AFLP, SSR, RFLP, and RAPD markers, and comparison with other sorghum maps. Theor. Appl. Genet. 105, 629–637.

    Article  PubMed  CAS  Google Scholar 

  • Kamatar M. Y. and Salimath P. M. 2003 Morphological traits of sorghum associated with resistance to shoot fly, Atherigona soccata Rondani. Ind. J. Plant Prot. 31, 73–77.

    Google Scholar 

  • Karanjkar R. R., Chundurwar R. D. and Borikar S. T. 1992 Correlation and path analysis of shoot fly resistance in sorghum. J. Maharashtra Agric. Univ. 17, 389–391.

    Google Scholar 

  • Kosambi D. D. 1944 The estimation of map distances from recombination values. Ann. Eugen. 12, 172–175.

    Article  Google Scholar 

  • Kumar A. A., Reddy B. V. S., Sharma H. C. and Ramaiah B. 2008 Shoot fly (Atherigona soccata) resistance in improved grain sorghum hybrids. E-Journal of SAT Agric. Res. 6, 1–4. doi:10.3914/lCRISAT.0039.

    Google Scholar 

  • Lander E. S., Green P., Abrahamson J., Barlow A. and Daly M. J. 1987 MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.

    Article  PubMed  CAS  Google Scholar 

  • Michelmore W. R., Paran I. and Kesseli R. V. 1991 Identification of marker linked to diseases resistance genes by bulked segregant analysis, a rapid method to detect the markers in specific genetic region by using the segregating population. Proc. Natl. Acad. Sci. USA 88, 9828–9832.

    Article  PubMed  CAS  Google Scholar 

  • Nwaze K. F. 1997 Screening for resistance to sorghum shoot fly. In Plant resistance to insect in sorghum. (ed. H. C. Sharma, F. Singh and K. F. Nwaze), pp. 35–37. International Crops Research Institute for the Semi- Arid Tropics, Patancheru, India.

  • Nwaze K. F., Reddy Y. V. R. and Soman P. 1990 The role of leaf surface wetness in larval behaviour of the sorghum shoot fly Atherigona soccata. Entomol. Exp. Appl. 56, 187–195.

    Article  Google Scholar 

  • Patel G. M. and Sukhani T. R. 1990 Biophysical plant characters associated with shoot fly resistance. Indian J. Ent. 52, 14–17.

    Google Scholar 

  • Sajjanar G. M. 2002 Genetic analysis and molecular mapping of components of resistance to shoot fly (Atherigona soccata) in sorghum (Sorghum bicolor (L.) Moench). Ph.D. thesis, University of Agricultural Sciences, Dharwad, India.

  • Satish K., Srinivas G., Madhusudhana R., Padmaja P. G., Reddy R. N., Mohan S. M. and Seetharama N. 2009 Identification of quantitative trait loci for resistance to shoot fly in sorghum (Sorghum bicolor (L.) Moench). Theor. Appl. Genet. 119, 1425–1439.

    Article  PubMed  CAS  Google Scholar 

  • Sharma H. C. 1997 Plant resistance to insects: Basic principles. In Plant resistance to insects in sorghum (ed. H. C. Sharma, F. Singh and K. F. Nwanze) pp. 24–31. ICRISAT, Patancheru, India.

    Google Scholar 

  • Venkateswarlu D. 2009 Marker-trait association study for shoot fly tolerance in sorghum using SSR markers. M.Sc. thesis, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India.

  • Wang S., Basten C. J. and Zeng Z. B. 2007 Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, USA (http://statger.ncsu.edu/qtlcart/WQTLCart.htm).

Download references

Author information

Authors and Affiliations

  1. Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola, 444 104, India

    D. B. APOTIKAR, D. VENKATESWARLU & P. L. KULWAL

  2. Sorghum Research Unit, Dr. Panjabrao Deshmukh Agricultural University, Akola, 444 104, India

    R. B. GHORADE

  3. Pulses Research Unit, Dr. Panjabrao Deshmukh Agricultural University, Akola, 444 104, India

    R. M. WADASKAR

  4. State Level Biotechnology Centre, Mahatma Phule Agricultural University, Rahuri, 413 722, India

    J. V. PATIL & P. L. KULWAL

Authors
  1. D. B. APOTIKAR
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. VENKATESWARLU
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. B. GHORADE
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. M. WADASKAR
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. V. PATIL
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. L. KULWAL
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. L. KULWAL.

Additional information

[Apotikar D. B., Venkateswarlu D., Ghorade R. B., Wadaskar R. M., Patil J. V. and Kulwal P. L. 2011 Mapping of shoot fly tolerance loci in sorghum using SSR markers. J. Genet. 90, 59–66]

These authors contributed equally to this work (D. B. Apotikar and D. Venkateswarlu).

Electronic supplementary material

Below is the link to the electronic supplementary material.

(PDF 510 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

APOTIKAR, D.B., VENKATESWARLU, D., GHORADE, R.B. et al. Mapping of shoot fly tolerance loci in sorghum using SSR markers. J Genet 90, 59–66 (2011). https://doi.org/10.1007/s12041-011-0046-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12041-011-0046-1

Keywords

Search

Navigation