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Molecular markers in management of ex situ PGR – A case study

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Abstract

Worldwide germplasm collections contain about 7.4 million accessions of plant genetic resources for food and agriculture. One of the 10 largest ex situ genebanks of our globe is located at the Leibniz Institute of Plant Genetics and Crop Plant Research in Gatersleben, Germany. Molecular tools have been used for various gene bank management practices including characterization and utilization of the germplasm. The results on genetic integrity of long-term-stored gene bank accessions of wheat (self-pollinating) and rye (open-pollinating) cereal crops revealed a high degree of identity for wheat. In contrast, the out-pollinating accessions of rye exhibited shifts in allele frequencies. The genetic diversity of wheat and barley germplasm collected at intervals of 40 to 50 years in comparable geographical regions showed qualitative rather than a quantitative change in diversity. The inter- and intraspecific variation of seed longevity was analysed and differences were detected. Genetic studies in barley, wheat and oilseed rape revealed numerous QTL, indicating the complex and quantitative nature of seed longevity. Some of the loci identified were in genomic regions that co-localize with genes determining agronomic traits such as spike architecture or biotic and abiotic stress response. Finally, a genome-wide association mapping analysis of a core collection of wheat for flowering time was performed using diversity array technology (DArT) markers. Maker trait associations were detected in genomic regions where major genes or QTL have been described earlier. In addition, new loci were also detected, providing opportunities to monitor genetic variation for crop improvement.

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Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Gatersleben, Germany

    Andreas Börner, Manuela Nagel, Mian Abdur Rehman Arif, Kerstin Neumann, Ulrike Lohwasser & Marion S Röder

  2. Institute of Cytology and Genetics (ICG), Siberian Branch of the Russian Academy of Sciences, Lavrentjeva Ave. 10, Novosibirsk, 630090, Russian Federation

    Elena K Khlestkina

  3. South Plant Biotechnology Center, Ovidiopolskaya dor. 3, 65036, Odessa, Ukraine

    Sabina Chebotar

  4. Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000, Novi Sad, Serbia

    Borislav Kobiljski

Authors
  1. Andreas Börner
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  2. Elena K Khlestkina
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  3. Sabina Chebotar
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  4. Manuela Nagel
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  5. Mian Abdur Rehman Arif
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  6. Kerstin Neumann
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  7. Borislav Kobiljski
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  8. Ulrike Lohwasser
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  9. Marion S Röder
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Corresponding author

Correspondence to Andreas Börner.

Additional information

[Börner A, Khlestkina EK, Chebotar S, Nagel M, Arif MAR, Neumann K, Kobiljski B, Lohwasser U and Röder MS 2012 Molecular markers in management of ex situ PGR – A case study. J. Biosci. 37 1–7] DOI 10.1007/s12038-012-9250-2

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Börner, A., Khlestkina, E.K., Chebotar, S. et al. Molecular markers in management of ex situ PGR – A case study. J Biosci 37, 871–877 (2012). https://doi.org/10.1007/s12038-012-9250-2

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  • DOI: https://doi.org/10.1007/s12038-012-9250-2

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