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Inactivation of a transgene due to transposition of insertion sequence (IS136) of Agrobacterium tumefaciens

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Abstract

Agrobacterium strains harbour insertion sequences, which are known to transpose into genomes as well as into Ti plasmids. In this study we report the inactivation of a transgene due to transposition of the A. tumefaciens insertion sequence IS136. The transposition was discovered following transformation of plant tissues, although the fidelity of the binary vector was confirmed following transformation into Agrobacterium. Such transpositions are rare but can occur and it is thus important to check the fidelity of the binary vector at different times of Agrobacterium growth in order to avoid failure in achieving transgene expression.

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Abbreviations

ELISA:

enyme-linked immunosorbent assay

IS:

insertion sequence

RT-PCR:

reverse transcriptase polymerase chain reaction

SBTI:

soyabean trypsin inhibitor (Kunitz type)

MS:

Murashige and Skoog

2,4-D:

2,4-dichlorophenoxyacetic acid

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Author notes

  1. Sanjeev Kumar

    Present address: Amity Institute of Biotechnology, Amity University, Noida, 201303, India

Authors and Affiliations

  1. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India

    Preeti Rawat, Sanjeev Kumar, Deepak Pental & Pradeep Kumar Burma

  2. Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India

    Deepak Pental

Authors
  1. Preeti Rawat
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  2. Sanjeev Kumar
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  3. Deepak Pental
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  4. Pradeep Kumar Burma
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Corresponding author

Correspondence to Pradeep Kumar Burma.

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Rawat, P., Kumar, S., Pental, D. et al. Inactivation of a transgene due to transposition of insertion sequence (IS136) of Agrobacterium tumefaciens . J Biosci 34, 199–202 (2009). https://doi.org/10.1007/s12038-009-0023-5

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  • DOI: https://doi.org/10.1007/s12038-009-0023-5

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