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Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation

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Abstract

Southern hybridisation was performed on ninety-six transgenic petunias that had been selected for resistance to kanamycin. Just over half of the plants contained intact copies of the T-DNA. The most common rearrangements (at least 24 plants out of 96) were simple deleted derivatives that had lost one or both ends of the T-DNA. T-DNAs lacking the left border occurred at a frequency of 20%, and estimates of the frequency of T-DNAs lacking the right border were at least this high. Three plants contained grossly rearranged T-DNAs, of which all expressed the kanamycin resistance gene but only one transmitted the gene to progeny. Two plants lacked T-DNA homology altogether and did not express kanamycin resistance in their leaves or their progeny. Circumstantial evidence suggests that plants containing a chimaeric kanamycin resistance gene driven by the ocs promoter do not root efficiently in the presence of kanamycin. There was no correlation between intactness of the T-DNA and Mendelian inheritance of the kanamycin-resistance phenotype. However, a disproportionate number of plants showing non-Mendelian inheritance had a high copy number of their T-DNA.

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  1. Department of Cellular and Molecular Biology, University of Auckland, Private Bag, Auckland, New Zealand

    Simon C. Deroles & Richard C. Gardner

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  1. Simon C. Deroles
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  2. Richard C. Gardner
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Deroles, S.C., Gardner, R.C. Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation. Plant Mol Biol 11, 365–377 (1988). https://doi.org/10.1007/BF00027393

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  • DOI: https://doi.org/10.1007/BF00027393

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