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Production of paclitaxel by Fusarium solani isolated from Taxus celebica

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Abstract

A fungus was isolated from the stem cuttings of Taxus celebica, which produced paclitaxel in liquid-grown cultures. The fungus was identified as Fusarium solani based on colony characteristics, morphology of conidia and the 26S rDNA sequence. Paclitaxel was identified by chromatographic and spectroscopic comparison with authentic paclitaxel and its cytotoxic activity towards Jurkat cells in vitro.

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Abbreviations

HPLC:

high-performance liquid chromatography

ITS:

internal transcribed spacer

LC-ESI-MS:

liquid chromatography electrospray ionization mass spectra

MALDI:

matrix-assisted laser desorption/ionization

PBS:

phosphate buffered saline

PCA:

potato carrot agar

PCR:

polymerase chain reaction

PDA:

potato dextrose agar

Rf:

Relative front

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Department of Biochemistry, Indian Institute of Science, Bangalore, 560 012, India

    B. V. S. K. Chakravarthi, Kalpana Surendranath, Anjali A. Karande & Chelliah Jayabaskaran

  2. Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, 560 012, India

    Prasanta Das

Authors
  1. B. V. S. K. Chakravarthi
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  2. Prasanta Das
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  3. Kalpana Surendranath
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  4. Anjali A. Karande
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  5. Chelliah Jayabaskaran
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Correspondence to Chelliah Jayabaskaran.

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Chakravarthi, B.V.S.K., Das, P., Surendranath, K. et al. Production of paclitaxel by Fusarium solani isolated from Taxus celebica . J Biosci 33, 259–267 (2008). https://doi.org/10.1007/s12038-008-0043-6

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  • DOI: https://doi.org/10.1007/s12038-008-0043-6

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