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The Efficacy Evaluation of Cryosurgery in Pancreatic Cancer Patients with the Expression of CD44v6, Integrin-β1, CA199, and CEA

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Abstract

Increased expression of cell adhesion molecule CD44v6, integrin-β1, carbohydrate antigen 199 (CA199), and carcinoembryonic antigen (CEA) are closely associated with the progression and metastasis of numerous cancers. In this study, peripheral blood mononuclear cell (PBMC) and serum samples were collected from 37 pancreatic cancer patients and 12 healthy people. A novel triplex TaqMan real-time reverse transcription polymerase chain reaction assay was used to measure the expression levels of CD44v6 and integrin-β1 gene in PBMCs, while chemiluminescence and enzyme-linked immunosorbent assay were used to measure the levels of CA199 and CEA expression in serum. The results showed that both the levels of CD44v6 and integrin-β1 expression had significant correlation with clinical stage, lymph node, and liver metastasis of pancreatic cancer (P < 0.05). Age, tumor size, tumor differentiation, clinical stage, lymph nodes, and liver metastasis were significantly associated with the levels of CA199 and CEA expression (P < 0.05). The levels of CD44v6, integrin-β1, CA199, and CEA expression in the patients prior cryosurgery and chemotherapy were significantly higher than those in the control group (P < 0.05), whereas no significant difference was found between the patients 1 month post cryosurgery and control group (P > 0.05). The expression levels of CD44v6, integrin-β1, CA199, and CEA in the patients 1 month post cryosurgery were significantly lower than those in the patients prior cryosurgery (P < 0.05). Interestingly, no significant difference was found for the CD44v6, integrin-β1, CA199, and CEA levels between the patients prior and post-chemotherapy (P > 0.05). The higher expression of CD44v6, integrin-β1, CA199, and CEA are closely related to the progression and metastasis of pancreatic cancer and may play a important role in the curative evaluation of cryosurgery of pancreatic cancer.

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Acknowledgments

We would like to express appreciation to Xiaopeng Liu, Zhiju Zhao, Chengqian Feng, and Mengtian Liao for processing clinical samples. The authors also thank the assistance of all medical and nursing staff of Fuda Cancer Hospital who cared for the patients in the study.

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Authors and Affiliations

  1. Department of Oncology, The GIBH Affiliated Fuda Hospital, Chinese Academy of Sciences, 91 JuDe Zhong Road, Chigang, Guangzhou, 510305, China

    Gang Zhou, David Chiu, Lizhi Niu, Lihua He & Kecheng Xu

  2. Stem Cell Research Group, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kai Yuan Avenue, Science Park, Guangzhou, 510610, China

    Dajiang Qin, Jinlei Cai & Wenhao Huang

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  1. Gang Zhou
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  2. David Chiu
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  3. Dajiang Qin
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  6. Lihua He
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Correspondence to Kecheng Xu.

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Zhou, G., Chiu, D., Qin, D. et al. The Efficacy Evaluation of Cryosurgery in Pancreatic Cancer Patients with the Expression of CD44v6, Integrin-β1, CA199, and CEA. Mol Biotechnol 52, 59–67 (2012). https://doi.org/10.1007/s12033-011-9474-7

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  • DOI: https://doi.org/10.1007/s12033-011-9474-7

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