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Enhanced Extracellular Production of Heterologous Proteins in Bacillus subtilis by Deleting the C-terminal Region of the SecA Secretory Machinery

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An Erratum to this article was published on 16 July 2010

Abstract

In this study, we examined the effects of modifying the C-terminal region of the SecA protein on the production of heterologous proteins in Bacillus subtilis. SecA was selected as a candidate among the components of the Sec system due to its ability to interact directly with both the precursors and membrane translocases. A phylogenetic comparison demonstrated that the C-terminal region is not well conserved among eubacterial SecA proteins. The deletion of the 61 amino acids at the C-terminal region led to an 83% increase in extracellular alkaliphilic Bacillus sp. thermostable alkaline cellulase (Egl-237) activity. Moreover, the productivity of human interferon α (hIFN-α2b) was increased by 2.2-fold compared to the wild-type SecA, by deletion of these 61 amino acids. We indicated that the deletion of the C-terminal domain (CTD) of SecA enhanced the secretion of two different heterologous protein, Egl-237 and hIFN-α2b. This study provides a useful method to enhance the extracellular production of heterologous proteins in B. subtilis.

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Acknowledgments

This study is the subproject, ‘Development of a Technology for Creation of a Host Cell’ included within the industrial technology project, ‘Development of a Generic Technology for Production Process Starting Productive Function’ of the Ministry of Economy, Trade and Industry (METI), entrusted by the New Energy and Industrial Technology Development Organization (NEDO), Japan.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8572, Japan

    Hiroshi Kakeshtia & Kouji Nakamura

  2. Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi, 321-3497, Japan

    Hiroshi Kakeshtia, Yasushi Kageyama, Katsutoshi Ara & Katsuya Ozaki

Authors
  1. Hiroshi Kakeshtia
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  2. Yasushi Kageyama
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  3. Katsutoshi Ara
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  4. Katsuya Ozaki
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  5. Kouji Nakamura
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Correspondence to Hiroshi Kakeshtia.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s12033-010-9313-2

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Kakeshtia, H., Kageyama, Y., Ara, K. et al. Enhanced Extracellular Production of Heterologous Proteins in Bacillus subtilis by Deleting the C-terminal Region of the SecA Secretory Machinery. Mol Biotechnol 46, 250–257 (2010). https://doi.org/10.1007/s12033-010-9295-0

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