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Infection and integration of high-risk human papillomavirus in HPV-associated cancer cells

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Abstract

High-risk human papillomaviruses (HPV) have been associated with many human cancers in clinical studies. Integration of HPV into the human genome is a suspected etiological factor in the induction of some HPV-associated cancers. The characteristics of HPV integration in certain HPV-integrated cancer cells remain unclear. In this study, ten HPV-associated carcinoma cell lines were evaluated for the presence, genotype, and integration status of HPV by nested polymerase chain reaction. The HPV genome did not insert in the genome of a mammary cancer cell line (MCF7), adrenal neuroblastoma cell line (NH-6), or three esophageal carcinoma cell lines (KYSE150, KYSE450 and KYSE140). HPV type 18 DNA did infect cell lines of tongue cancer (Tca83), hepatocellular carcinoma (Hep G2), and lung carcinoma (A549), but the HPV type 18 genes were not transcribed into mRNA. However, HPV type 18 integrated into the genomes of the esophageal carcinoma cell lines EC9706 and EC109, and the integration sites for both cell lines were in loci 8q24, which is a gene desert area adjacent to fragile sites. We speculate that HPV transcripts are more likely to integrate near highly susceptible fragile sites. This study suggests that HPV integration is still a significant issue that needs to be fully examined and can possibly be used as individualized biomarkers for the early diagnosis of HPV-related cancers.

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Acknowledgments

This study was supported by National Natural Sciences Foundation of China (No. 21277001) and Beijing Municipal Education Commission Science and Technology Project (No. KZ201110005003), partially supported by The State Key Laboratories Development Program of China (2011SLKID103) and the National High Technology Research Program of China (863 program) (Grant No. 2012AA02A404).

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All the authors declare no competing financial interests.

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Authors and Affiliations

  1. Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China

    Chu-Yi Liu, Fan Li, Yi Zeng, Min-zhong Tang, Jin-Tao Li & Ru-Gang Zhong

  2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Beijing, 100052, China

    Yi Zeng & Jin-Tao Li

  3. Wuzhou Red Cross Hospital, Wuzhou, China

    Min-zhong Tang & Yulu Huang

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  1. Chu-Yi Liu
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  2. Fan Li
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  6. Jin-Tao Li
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Correspondence to Jin-Tao Li or Ru-Gang Zhong.

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Liu, CY., Li, F., Zeng, Y. et al. Infection and integration of high-risk human papillomavirus in HPV-associated cancer cells. Med Oncol 32, 109 (2015). https://doi.org/10.1007/s12032-015-0560-8

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  • DOI: https://doi.org/10.1007/s12032-015-0560-8

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