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Elevated expression patterns and tight correlation of the PLCE1 and NF-κB signaling in Kazakh patients with esophageal carcinoma

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Abstract

This study investigated the expression of the phospholipase C epsilon 1 (PLCE1) and nuclear factor-kappaB (NF-κB)-related proteins in Kazakh patients with esophageal squamous cell carcinoma (ESCC). Tissue microarrays of 90 ethnic Kazakh patients with ESCC and exhibiting clinical characteristics were analyzed for protein expression of PLCE1, IKKβ, IKBα, p50, and p65 by immunohistochemistry. Correlations between histoscores of PLCE1 and NF-κB-related proteins were determined using Spearman’s rank correlation tests. Expression of PLCE1 and NF-κB-related proteins significantly increased in tumor tissues compared with normal esophageal tissues (P = 9.48 × 10−7, 1.24 × 10−5, 0.004, 0.003, and 2.83 × 10−5, respectively). Upregulation of PLCE1 was significantly correlated with advanced tumor-node-metastasis stages (P = 0.018) and lymph node metastasis (P = 0.003). Overexpression of IKKβ and IKBα was associated with ESCC stages I/II (P = 3.36 × 10−4 and 0.022, respectively). Increased expression of p50 was significantly higher in patients with lymph node metastasis than without lymph node metastasis (P = 0.048). Elevated expression of p65 protein was significantly correlated with poor and moderately differentiated ESCC and depth of tumor invasion (P = 0.026 and 0.010, respectively). Significant positive correlations were observed between the expression of PLCE1 and NF-κB-related proteins, especially IKKβ (r = 0.246 and P = 0.025) and p50 (r = 0.244 and P = 0.024). These results suggest, for the first time, that upregulation of PLCE1 is correlated with increased expression of NF-κB-related proteins in Kazakh patients with ESCC, suggesting that interaction between PLCE1 with the NF-κB signal pathway may be responsible for the carcinogenesis of ESCC, such as ESCC-related inflammation.

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Acknowledgments

This study was supported by National Natural Science Foundation of China (81160301, 81360358, 81260301), Ministry of Science and Technology of China (Grant No. 2010DFB34100 and 2012AA02A503) and Maxin Pathology Fund of China (m1108).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, North 4th Road, Shihezi, 832002, Xinjiang, People’s Republic of China

    Xiao-bin Cui, Xue-lian Pang, Su Li, Jing Jin, Jian-ming Hu, Lan Yang, Chun-xia Liu, Wei-hua Liang, Yun-zhao Chen & Feng Li

  2. Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

    Xiao-bin Cui, Yun-zhao Chen & Feng Li

  3. Department of Pathology, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, People’s Republic of China

    Xiao-bin Cui, Li Li, Yun-zhao Chen & Feng Li

  4. Operating Room, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, People’s Republic of China

    Shu Jun Wen

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  1. Xiao-bin Cui
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  2. Xue-lian Pang
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  3. Su Li
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Correspondence to Yun-zhao Chen or Feng Li.

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Xiao-bin Cui and Xue-lian Pang have contributed equally to this work.

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Cui, Xb., Pang, Xl., Li, S. et al. Elevated expression patterns and tight correlation of the PLCE1 and NF-κB signaling in Kazakh patients with esophageal carcinoma. Med Oncol 31, 791 (2014). https://doi.org/10.1007/s12032-013-0791-5

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